Efficacy of biological activity of andrographis paniculata extracted by using supercritical carbon dioxide (Sc-CO2) extraction

• Main Authors: Rithwan, Fahim, Zhari, Salman, Che Yunus, Mohd. Azizi, Mohd. Hadzri, Hafiza
• Format: Article
• Language: English
• Published: Penerbit UTM 2014
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/52625/1/Mohd.AziziChe2014_Efficacyofbiologicalactivity.pdf; http://eprints.utm.my/id/eprint/52625/; https://dx.doi.org/10.11113/jt.v69.3175

Andrographis paniculata plant has been widely used for traditional medicine such as curing fever, diarrhoea, and inflammation. In this study, the main component (Andrographolide) is extracted from the leaves of A. paniculata using supercritical carbon dioxide (Sc-CO2) and Sc-CO2 assisted by methanol as a modifier solvent. The methanol soxhlet extraction as a standard method was used to compare with the Sc-CO2 extraction. This work also focuses on the determination of anti-oxidant activity of Sc-CO2 extraction extracts of A. paniculata plant. The Sc-CO2 extracts of A. paniculata for both Sc-CO2 extractions with and without modifier showed promising antioxidant activity. Free radical scavenging potential of both Sc-CO2 extractions of A. paniculata plant was evaluated by using reducing power. In this method, ascorbic acid was used as a standard for determining reducing power. Both Sc-CO2 extract of A. paniculata plant exhibited appreciable activity as compared to the methanol soxhlet extract, indicating that A. paniculata has promising free radical scavenging activity.