Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale
The process for high cell mass and spore production by Bacillus thuringiensis var israelensis (Bti) on semi-industrial scale was developed. Cultivations were conducted in shake flask and bioreactor levels to develop a semi-industrial scale process for the production of Bti cells and spores. The comp...
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Journal of Pure and Applied Microbiology
2014
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my.utm.520182018-11-30T07:00:20Z http://eprints.utm.my/id/eprint/52018/ Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale Elsayed, Elsayed Ahmed Othman, Nor Zalina Abd. Malek, Roslinda Awad, Hassan Mohamed Aziz, Md. Mijanur Wu, King Wadaan, Mohammad Ahmed El-Enshasy, Hesham Ali QH Natural history The process for high cell mass and spore production by Bacillus thuringiensis var israelensis (Bti) on semi-industrial scale was developed. Cultivations were conducted in shake flask and bioreactor levels to develop a semi-industrial scale process for the production of Bti cells and spores. The composition of the most suitable cultivation medium was further optimized to enhance biomass and spore production and also to reduce the production time. Furthermore, cultivations were performed in 16-L stirred tank bioreactor to investigate the scalability of this process and to study the effect of different aeration rates. The optimization of the main three components of the cultivation medium, i.e. glucose, ammonium sulphate and phosphate, resulted in a significant increase in both cell growth and spore formation by about 32% and 119%, respectively. Scaling up the cultivation process from shake flask to 16-L bioreactor further improved the process. Maximal cell mass of 16.06 g.L-1 concomitant with a spore production of 152x107 spores.mL-1 was achieved in lv.v-1.min-1 aerated culture. In terms of cell growth and spore production, these results were 5- and 8.5-fold higher, respectively, compared with the initial un-optimized medium in shake flask culture. The results obtained in this study improved the process of Bti production in semi-industrial scale through the optimization of both the cultivation medium composition and the aeration rate in pilot scale stirred tank bioreactor. Journal of Pure and Applied Microbiology 2014 Article PeerReviewed Elsayed, Elsayed Ahmed and Othman, Nor Zalina and Abd. Malek, Roslinda and Awad, Hassan Mohamed and Aziz, Md. Mijanur and Wu, King and Wadaan, Mohammad Ahmed and El-Enshasy, Hesham Ali (2014) Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale. Journal of Pure and Applied Microbiology, 8 (4). pp. 2773-2783. ISSN 0973-7510 https://microbiologyjournal.org |
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QH Natural history Elsayed, Elsayed Ahmed Othman, Nor Zalina Abd. Malek, Roslinda Awad, Hassan Mohamed Aziz, Md. Mijanur Wu, King Wadaan, Mohammad Ahmed El-Enshasy, Hesham Ali Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale |
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The process for high cell mass and spore production by Bacillus thuringiensis var israelensis (Bti) on semi-industrial scale was developed. Cultivations were conducted in shake flask and bioreactor levels to develop a semi-industrial scale process for the production of Bti cells and spores. The composition of the most suitable cultivation medium was further optimized to enhance biomass and spore production and also to reduce the production time. Furthermore, cultivations were performed in 16-L stirred tank bioreactor to investigate the scalability of this process and to study the effect of different aeration rates. The optimization of the main three components of the cultivation medium, i.e. glucose, ammonium sulphate and phosphate, resulted in a significant increase in both cell growth and spore formation by about 32% and 119%, respectively. Scaling up the cultivation process from shake flask to 16-L bioreactor further improved the process. Maximal cell mass of 16.06 g.L-1 concomitant with a spore production of 152x107 spores.mL-1 was achieved in lv.v-1.min-1 aerated culture. In terms of cell growth and spore production, these results were 5- and 8.5-fold higher, respectively, compared with the initial un-optimized medium in shake flask culture. The results obtained in this study improved the process of Bti production in semi-industrial scale through the optimization of both the cultivation medium composition and the aeration rate in pilot scale stirred tank bioreactor. |
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Article |
author |
Elsayed, Elsayed Ahmed Othman, Nor Zalina Abd. Malek, Roslinda Awad, Hassan Mohamed Aziz, Md. Mijanur Wu, King Wadaan, Mohammad Ahmed El-Enshasy, Hesham Ali |
author_facet |
Elsayed, Elsayed Ahmed Othman, Nor Zalina Abd. Malek, Roslinda Awad, Hassan Mohamed Aziz, Md. Mijanur Wu, King Wadaan, Mohammad Ahmed El-Enshasy, Hesham Ali |
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Elsayed, Elsayed Ahmed |
title |
Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale |
title_short |
Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale |
title_full |
Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale |
title_fullStr |
Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale |
title_full_unstemmed |
Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale |
title_sort |
bioprocess development for high cell mass and endospore production by bacillus thuringiensis var israelensis in semi-industrial scale |
publisher |
Journal of Pure and Applied Microbiology |
publishDate |
2014 |
url |
http://eprints.utm.my/id/eprint/52018/ https://microbiologyjournal.org |
_version_ |
1643653129241100288 |
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13.18916 |