The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media
One of the criteria for large scale production of therapeutics using animal cell lines is the ability to grow in and can be maintained in inexpensive, serum free defined medium. Serum free medium however, compromised by the high levels of cell death seen in bioreactors, which is mainly via apoptosis...
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Faculty of Chemical and Natural Resources Engineering, UTM
2001
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my.utm.50752010-06-01T03:23:16Z http://eprints.utm.my/id/eprint/5075/ The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media Abdul Majid, Fadzilah Adibah Al-Rubeai, Mohamed T Technology (General) One of the criteria for large scale production of therapeutics using animal cell lines is the ability to grow in and can be maintained in inexpensive, serum free defined medium. Serum free medium however, compromised by the high levels of cell death seen in bioreactors, which is mainly via apoptosis. Previous studies in our laboratory have demonstrated that apoptosis in bioreactors can be prevented by bcl-2 over-expression in these cell lines. The robustness of the bcl-2 transfected hybridoma (TB/C3) cell line could allow them to be grown in simple medium formulation especially without serum supplementation which could facilitate the down stream processing, overall manufacturing process and product consistency. In the present study, the impact of the suppression of apoptosis on cell adaptation to various media formulation was carried out. This cell line was successfully grown in suspension batch culture with WMC5, HybSFM, DMEM/F12, WMC5/RPMI 1640 media and in serial dilution of HybSFM without prior adaptation. Faculty of Chemical and Natural Resources Engineering, UTM 2001 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/5075/1/FadzilahAdibahAbdulMajid2001_TheEffectOfbcl-2OverExpression.pdf Abdul Majid, Fadzilah Adibah and Al-Rubeai, Mohamed (2001) The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media. Proceedings of The 15th Symposium of Malaysian Chemical Engineers SOMChE 2001 . pp. 524-530. |
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T Technology (General) Abdul Majid, Fadzilah Adibah Al-Rubeai, Mohamed The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
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One of the criteria for large scale production of therapeutics using animal cell lines is the ability to grow in and can be maintained in inexpensive, serum free defined medium. Serum free medium however, compromised by the high levels of cell death seen in bioreactors, which is mainly via apoptosis. Previous studies in our laboratory have demonstrated that apoptosis in bioreactors can be prevented by bcl-2 over-expression in these cell lines. The robustness of the bcl-2 transfected hybridoma (TB/C3) cell line could allow them to be grown in simple medium formulation especially without serum supplementation which could facilitate the down stream processing, overall manufacturing process and product consistency. In the present study, the impact of the suppression of apoptosis on cell adaptation to various media formulation was carried out. This cell line was successfully grown in suspension batch culture with WMC5, HybSFM, DMEM/F12, WMC5/RPMI 1640 media and in serial dilution of HybSFM without prior adaptation. |
format |
Article |
author |
Abdul Majid, Fadzilah Adibah Al-Rubeai, Mohamed |
author_facet |
Abdul Majid, Fadzilah Adibah Al-Rubeai, Mohamed |
author_sort |
Abdul Majid, Fadzilah Adibah |
title |
The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
title_short |
The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
title_full |
The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
title_fullStr |
The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
title_full_unstemmed |
The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
title_sort |
effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media |
publisher |
Faculty of Chemical and Natural Resources Engineering, UTM |
publishDate |
2001 |
url |
http://eprints.utm.my/id/eprint/5075/1/FadzilahAdibahAbdulMajid2001_TheEffectOfbcl-2OverExpression.pdf http://eprints.utm.my/id/eprint/5075/ |
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13.18916 |