Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study
Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aime...
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my.utm.505262018-09-30T08:17:14Z http://eprints.utm.my/id/eprint/50526/ Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study Chua, Kien Hui Lee, Ting Hun Nagandran, Kamini Md. Yahaya, Nor Hamdan Lee, Chew Tin Tan, Eddie Ti Tjih Abdul Aziz, Ramlan R Medicine Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.Results: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis 2013 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/50526/1/LeeTingHun2013_Ediblebird%27snestextract.pdf Chua, Kien Hui and Lee, Ting Hun and Nagandran, Kamini and Md. Yahaya, Nor Hamdan and Lee, Chew Tin and Tan, Eddie Ti Tjih and Abdul Aziz, Ramlan (2013) Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study. BMC Complementary and Alternative Medicine, 13 . ISSN 1472-6882 http://dx.doi.org/10.1186/1472-6882-13-19 DOI: 10.1186/1472-6882-13-19 |
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R Medicine Chua, Kien Hui Lee, Ting Hun Nagandran, Kamini Md. Yahaya, Nor Hamdan Lee, Chew Tin Tan, Eddie Ti Tjih Abdul Aziz, Ramlan Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
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Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.Results: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis |
format |
Article |
author |
Chua, Kien Hui Lee, Ting Hun Nagandran, Kamini Md. Yahaya, Nor Hamdan Lee, Chew Tin Tan, Eddie Ti Tjih Abdul Aziz, Ramlan |
author_facet |
Chua, Kien Hui Lee, Ting Hun Nagandran, Kamini Md. Yahaya, Nor Hamdan Lee, Chew Tin Tan, Eddie Ti Tjih Abdul Aziz, Ramlan |
author_sort |
Chua, Kien Hui |
title |
Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
title_short |
Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
title_full |
Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
title_fullStr |
Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
title_full_unstemmed |
Edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
title_sort |
edible bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study |
publishDate |
2013 |
url |
http://eprints.utm.my/id/eprint/50526/1/LeeTingHun2013_Ediblebird%27snestextract.pdf http://eprints.utm.my/id/eprint/50526/ http://dx.doi.org/10.1186/1472-6882-13-19 |
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