Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation

• Main Authors: Md. Noh, Abidah, Mohamad, Siti Hajar, Md. Jahim, Jamaliah, Abdul Murad, Abdul Munir, Abu Bakar, Farah Diba, Muhammad, Nor, Mahadi, Mahadi, Md. Illias, Rosli
• Format: Article
• Published: 2012
• Subjects: Q Science
• Online Access: http://eprints.utm.my/id/eprint/47403/; http://www.ajbasweb.com/old/ajbas/2012/January/43-49.pdf

This study was aimed to investigate the production of recombinant cellobiohydrolase (CBH) in static liquid culture (SLC) fermentation using newly developed recombinant of A. niger. Different environmental parameters and culture conditions were used to attain good cultivation of A. niger in SLC fermentation and highest level of CBH production. The fermentation results were evaluated from CBH production, total biomass, total protein and the residual main carbon source, maltose after 9 days of cultivation. The CBH production was found effectively enhanced under optimized cultivation temperature, initial pH, inoculums size and pre-shaked inoculums. Maximum biomass, protein concentration and enzyme activity were determined as 1.5 g dry weight/ml medium, 3.5 mg/ml and 40 U/ml respectively. The results also show that SLC could enhance production of the recombinant CBH.