Chemical compositions and antibacterial activity of the leaf and stem oils of piper porphyrophyllum (lindl.) N.E. Br.

• Main Authors: Wan Salleh, Wan Mohd. Nuzul Hakimi, Ahmad, Farediah, Mohd. Sirat, Hasnah, Khong, Heng Yen
• Format: Article
• Language: English
• Published: 2012
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/46688/1/W.M.W.H.WanSalleh_2012_Chemical%20compositions%20and%20antibacterial%20activity%20of%20the%20leaf%20and%20stem%20oils.pdf; http://eprints.utm.my/id/eprint/46688/

The essential oils obtained by hydrodistillation from the fresh leaf and stem of Piper porphyrophyllum N.E. Br. were analyzed by GC and GC/MS. Thirty four constituents were identified in the leaf oil, while thirty eight constituents were identified in the stems oil. The most abundant components in the leaf oil included bicyclogermacrene (14.7 %), α-copaene (13.2 %) and β-phellandrene (9.5 %) while sabinene (15.5 %), bicyclogermacrene (12.3 %) and α-copaene (8.1 %) were the main constituents in the stem oil. The evaluation of antibacterial activity by using micro-dilution method revealed that both oils were moderately active against all the Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Pseudomonas aeruginosa, Pseudomonas putida and Escherichia coli) with minimum inhibitory concentration (MIC) values in the range 125-1000 µg/ml.