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Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design

A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, am...

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Main Authors: Wahid, M. Z. A., Salleh, M., Yusof, F., Karim, M. I. A., Alam, Z.
Format: Article
Published: Academic Journals 2011
Subjects:
QK Botany
Online Access:http://eprints.utm.my/id/eprint/44923/
http://www.ajol.info/index.php/ajb/article/view/95683
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spelling my.utm.449232017-01-31T07:00:21Z http://eprints.utm.my/id/eprint/44923/ Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design Wahid, M. Z. A. Salleh, M. Yusof, F. Karim, M. I. A. Alam, Z. QK Botany A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p= 0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p= 0.023) have been recognized as significant factors affecting endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by T. reesei RUT C-30. The model established from the ANOVA analysis have a significant value of Pmodel>F= 0.0008 and R2 of 0.9132. The preoptimized media showed 2.6 fold increased of endoglucanase activity. Academic Journals 2011 Article PeerReviewed Wahid, M. Z. A. and Salleh, M. and Yusof, F. and Karim, M. I. A. and Alam, Z. (2011) Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design. African Journal of Biotechnology, 10 (46). pp. 9402-9409. ISSN 1684-5315 http://www.ajol.info/index.php/ajb/article/view/95683
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QK Botany
spellingShingle QK Botany
Wahid, M. Z. A.
Salleh, M.
Yusof, F.
Karim, M. I. A.
Alam, Z.
Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design
description A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p= 0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p= 0.023) have been recognized as significant factors affecting endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by T. reesei RUT C-30. The model established from the ANOVA analysis have a significant value of Pmodel>F= 0.0008 and R2 of 0.9132. The preoptimized media showed 2.6 fold increased of endoglucanase activity.
format Article
author Wahid, M. Z. A.
Salleh, M.
Yusof, F.
Karim, M. I. A.
Alam, Z.
author_facet Wahid, M. Z. A.
Salleh, M.
Yusof, F.
Karim, M. I. A.
Alam, Z.
author_sort Wahid, M. Z. A.
title Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design
title_short Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design
title_full Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design
title_fullStr Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design
title_full_unstemmed Factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman Design
title_sort factors affecting endoglucase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using plackett-burman design
publisher Academic Journals
publishDate 2011
url http://eprints.utm.my/id/eprint/44923/
http://www.ajol.info/index.php/ajb/article/view/95683
_version_ 1643651587595304960
score 13.214268

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