Fabrication and application of nanofork for measuring single cells adhesion force inside ESEM
In this paper, single cell adhesion force was measured using a nanofork. The nanofork was used to pick-up a single cell on a line-patterned substrate inside ESEM. The line-patterned substrate was used to provide small gaps between the single cells and the substrate. Therefore, the nanofork could be...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Institute of Electrical and Electronics Engineers
2011
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/44921/ |
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| Summary: | In this paper, single cell adhesion force was measured using a nanofork. The nanofork was used to pick-up a single cell on a line-patterned substrate inside ESEM. The line-patterned substrate was used to provide small gaps between the single cells and the substrate. Therefore, the nanofork could be inserted through these gaps in order to successfully pick-up a single cell. Adhesion force was measured during the cell pick-up process from the deflection of the cantilever beam. The nanofork was fabricated using focused ion beam (FIB) etching process while the line-patterned substrate was fabricated using nanoimprinting technology. As to investigate the effect of contact area on the strength of the adhesion force, two sizes of gap distance of line-patterned substrate were used, i.e. 1 µm and 2 µm. Results showed that cells attached on the 1 µm gap line-patterned substrate required more force to be released as compared to the cells attached on the 2 µm gap line-patterned substrate. |
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