Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes
In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher t...
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my.utm.366752017-11-01T04:17:19Z http://eprints.utm.my/id/eprint/36675/ Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes Abdul Aziz, Azila Jusoh, Norhana Ashari, Mohd. Azizul Hassan, Norhasliza TP Chemical technology In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher the PVA concentration used for immobilization, the better the retention of the enzyme in the membrane. Nevertheless, higher PVA concentration did not necessarily correlate well with enzyme activity. Freeze–thawed PVA–GOD membranes with 10% PVA showed the highest sensitivity to glucose. The performances of freeze–thawed PVA–GOD membranes with 15% and 5% PVA was comparable. For the second immobilization method, GOD was immobilized within PVA/tetramethoxysilane (TMOS) matrix with different (3–glycidoxypropyldimenthylethoxy) silane (3GPDES) concentrations. For the membranes prepared with 1:1 (TMOS: 3GPDES) volumetric ratio, the percentage of enzyme activity remaining at day 40 was about 51%. Meanwhile, membrane prepared with 1:2 (TMOS: 3GPDES) and 1:3 (TMOS: 3GPDES) were quite stable with 69% and 58% of enzyme activity remaining at day 40, respectively. Overall, the glucose biosensor showed very promising performances. Penerbit UTM Press 2010-05 Article PeerReviewed text/html en http://eprints.utm.my/id/eprint/36675/2/121 application/pdf en http://eprints.utm.my/id/eprint/36675/3/AzilaAbdulAziz2010_ImmobilizationofGlucoseOxidaseinFreeze.pdf Abdul Aziz, Azila and Jusoh, Norhana and Ashari, Mohd. Azizul and Hassan, Norhasliza (2010) Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes. Jurnal Teknologi, 52 . pp. 181-192. ISSN 0127-9696 (Print); 2180-3722 (Electronic) DOI:10.11113/jt.v52.131 |
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TP Chemical technology Abdul Aziz, Azila Jusoh, Norhana Ashari, Mohd. Azizul Hassan, Norhasliza Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes |
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In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher the PVA concentration used for immobilization, the better the retention of the enzyme in the membrane. Nevertheless, higher PVA concentration did not necessarily correlate well with enzyme activity. Freeze–thawed PVA–GOD membranes with 10% PVA showed the highest sensitivity to glucose. The performances of freeze–thawed PVA–GOD membranes with 15% and 5% PVA was comparable. For the second immobilization method, GOD was immobilized within PVA/tetramethoxysilane (TMOS) matrix with different (3–glycidoxypropyldimenthylethoxy) silane (3GPDES) concentrations. For the membranes prepared with 1:1 (TMOS: 3GPDES) volumetric ratio, the percentage of enzyme activity remaining at day 40 was about 51%. Meanwhile, membrane prepared with 1:2 (TMOS: 3GPDES) and 1:3 (TMOS: 3GPDES) were quite stable with 69% and 58% of enzyme activity remaining at day 40, respectively. Overall, the glucose biosensor showed very promising performances. |
format |
Article |
author |
Abdul Aziz, Azila Jusoh, Norhana Ashari, Mohd. Azizul Hassan, Norhasliza |
author_facet |
Abdul Aziz, Azila Jusoh, Norhana Ashari, Mohd. Azizul Hassan, Norhasliza |
author_sort |
Abdul Aziz, Azila |
title |
Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes |
title_short |
Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes |
title_full |
Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes |
title_fullStr |
Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes |
title_full_unstemmed |
Immobilization of glucose oxidase in freeze - thawed PVa and PVa/TMOS membranes |
title_sort |
immobilization of glucose oxidase in freeze - thawed pva and pva/tmos membranes |
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Penerbit UTM Press |
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2010 |
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http://eprints.utm.my/id/eprint/36675/2/121 http://eprints.utm.my/id/eprint/36675/3/AzilaAbdulAziz2010_ImmobilizationofGlucoseOxidaseinFreeze.pdf http://eprints.utm.my/id/eprint/36675/ |
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13.18916 |