Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme
Reverse micelle extraction by using Sodium bis (2-ethylhexyl) Suffoccinate (AOT) of protein bovine serum albumin (BSA) and lysozyme was investigated in this research. Study of factors affecting the surfactant concentration and pH of aqueous for both forward and backward extraction process was perfor...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Penerbit UTM Press
2012
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/33062/1/SitiHamidahMohdSetapar2012_ReverseMicelleLiquidLiquidExtraction.pdf http://eprints.utm.my/id/eprint/33062/ http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/1256 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.utm.33062 |
---|---|
record_format |
eprints |
spelling |
my.utm.330622017-11-01T04:17:14Z http://eprints.utm.my/id/eprint/33062/ Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme Mohd. Setapar, Siti Hamidah Mohamad Aziz, Siti Norazimah Joanne, Constant QD Chemistry Reverse micelle extraction by using Sodium bis (2-ethylhexyl) Suffoccinate (AOT) of protein bovine serum albumin (BSA) and lysozyme was investigated in this research. Study of factors affecting the surfactant concentration and pH of aqueous for both forward and backward extraction process was performed in the research. The BSA concentrations were characterized by using the UV- spectrophotometer at wavelength, ? = 280 nm. The result indicated that the extraction percentage of lysozyme was higher than BSA in forward transfer for both parameters; however BSA demonstrated a better extraction performance in backward extraction process. The maximum lysozyme extracted in the forward extraction process was at 60 mM of surfactant concentration while for BSA was 100 mM since BSA is a bulky molecule and the size is larger than of lysozyme. Penerbit UTM Press 2012 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/33062/1/SitiHamidahMohdSetapar2012_ReverseMicelleLiquidLiquidExtraction.pdf Mohd. Setapar, Siti Hamidah and Mohamad Aziz, Siti Norazimah and Joanne, Constant (2012) Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme. Jurnal Teknologi, 58 . pp. 7-11. ISSN 2180-3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/1256 |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
language |
English |
topic |
QD Chemistry |
spellingShingle |
QD Chemistry Mohd. Setapar, Siti Hamidah Mohamad Aziz, Siti Norazimah Joanne, Constant Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
description |
Reverse micelle extraction by using Sodium bis (2-ethylhexyl) Suffoccinate (AOT) of protein bovine serum albumin (BSA) and lysozyme was investigated in this research. Study of factors affecting the surfactant concentration and pH of aqueous for both forward and backward extraction process was performed in the research. The BSA concentrations were characterized by using the UV- spectrophotometer at wavelength, ? = 280 nm. The result indicated that the extraction percentage of lysozyme was higher than BSA in forward transfer for both parameters; however BSA demonstrated a better extraction performance in backward extraction process. The maximum lysozyme extracted in the forward extraction process was at 60 mM of surfactant concentration while for BSA was 100 mM since BSA is a bulky molecule and the size is larger than of lysozyme. |
format |
Article |
author |
Mohd. Setapar, Siti Hamidah Mohamad Aziz, Siti Norazimah Joanne, Constant |
author_facet |
Mohd. Setapar, Siti Hamidah Mohamad Aziz, Siti Norazimah Joanne, Constant |
author_sort |
Mohd. Setapar, Siti Hamidah |
title |
Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
title_short |
Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
title_full |
Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
title_fullStr |
Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
title_full_unstemmed |
Reverse Micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
title_sort |
reverse micelle liquid-liquid extraction of bovine serum albumin and lysozyme |
publisher |
Penerbit UTM Press |
publishDate |
2012 |
url |
http://eprints.utm.my/id/eprint/33062/1/SitiHamidahMohdSetapar2012_ReverseMicelleLiquidLiquidExtraction.pdf http://eprints.utm.my/id/eprint/33062/ http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/1256 |
_version_ |
1643649219016261632 |
score |
13.160551 |