Successful plant regeneration of Orthosiphon stamineus from petiole
We present a successful regeneration of Orthosiphon stamineus plant from petiole. All of the explants were cultured on Murashige and Skoog (MS)-based medium supplemented with various concentrations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) at the growth condition (25 ± 2°C, 16-h p...
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my.utm.330402019-03-05T02:54:31Z http://eprints.utm.my/id/eprint/33040/ Successful plant regeneration of Orthosiphon stamineus from petiole Mohd. Nawi, I. H. Abd. Samad, Azman Q Science We present a successful regeneration of Orthosiphon stamineus plant from petiole. All of the explants were cultured on Murashige and Skoog (MS)-based medium supplemented with various concentrations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) at the growth condition (25 ± 2°C, 16-h photo period at light intensity of 22.85 μmol /m2 /s) for 8 weeks. Results showed that petiole explants cultured at combination of 1.0 mg/L BAP and 0.2 mg/L NAA gave highest number of shoot produced per explants (4.33 ± 0.33) and a maximum callus fresh weight gains (3.2 ± 1.9 g) as compared to other treatments. The first shoot was observed from petiole cultured at all treatments in 4 weeks culture except for 0.1 mg/L BAP + 0.5 mg/L NAA treatment. For root induction, treatment at 0.1 mg/L NAA produced the highest number of roots produced per shoot (9.8 ± 3.1) compared to other treatments. Regeneration comparison between petiole, young leaf and stem segments showed that petiole is the most suitable explant for an efficient plant regeneration system of O. stamineus. Academic Journals 2012-07 Article PeerReviewed Mohd. Nawi, I. H. and Abd. Samad, Azman (2012) Successful plant regeneration of Orthosiphon stamineus from petiole. Journal of Medicinal Plants Research, 6 (26). pp. 4276-4280. ISSN 1996-0875 http://www.academicjournals.org/article/article1380725270_Nawi%20and%20Samad.pdf |
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Q Science Mohd. Nawi, I. H. Abd. Samad, Azman Successful plant regeneration of Orthosiphon stamineus from petiole |
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We present a successful regeneration of Orthosiphon stamineus plant from petiole. All of the explants were cultured on Murashige and Skoog (MS)-based medium supplemented with various concentrations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) at the growth condition (25 ± 2°C, 16-h photo period at light intensity of 22.85 μmol /m2 /s) for 8 weeks. Results showed that petiole explants cultured at combination of 1.0 mg/L BAP and 0.2 mg/L NAA gave highest number of shoot produced per explants (4.33 ± 0.33) and a maximum callus fresh weight gains (3.2 ± 1.9 g) as compared to other treatments. The first shoot was observed from petiole cultured at all treatments in 4 weeks culture except for 0.1 mg/L BAP + 0.5 mg/L NAA treatment. For root induction, treatment at 0.1 mg/L NAA produced the highest number of roots produced per shoot (9.8 ± 3.1) compared to other treatments. Regeneration comparison between petiole, young leaf and stem segments showed that petiole is the most suitable explant for an efficient plant regeneration system of O. stamineus. |
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Article |
author |
Mohd. Nawi, I. H. Abd. Samad, Azman |
author_facet |
Mohd. Nawi, I. H. Abd. Samad, Azman |
author_sort |
Mohd. Nawi, I. H. |
title |
Successful plant regeneration of Orthosiphon stamineus from petiole |
title_short |
Successful plant regeneration of Orthosiphon stamineus from petiole |
title_full |
Successful plant regeneration of Orthosiphon stamineus from petiole |
title_fullStr |
Successful plant regeneration of Orthosiphon stamineus from petiole |
title_full_unstemmed |
Successful plant regeneration of Orthosiphon stamineus from petiole |
title_sort |
successful plant regeneration of orthosiphon stamineus from petiole |
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Academic Journals |
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2012 |
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http://eprints.utm.my/id/eprint/33040/ http://www.academicjournals.org/article/article1380725270_Nawi%20and%20Samad.pdf |
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13.209306 |