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Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)

An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected w...

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Main Authors: Yap, Wei Ney, Abdul-Rahman, Badarulhisam, Abdul-Aziz, Azila
Format: Conference or Workshop Item
Language:English
Published: 2004
Subjects:
QH301 Biology
Online Access:http://eprints.utm.my/id/eprint/3063/1/50_%28C5-4%29.pdf
http://eprints.utm.my/id/eprint/3063/
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spelling my.utm.30632017-08-30T07:03:16Z http://eprints.utm.my/id/eprint/3063/ Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) Yap, Wei Ney Abdul-Rahman, Badarulhisam Abdul-Aziz, Azila QH301 Biology An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected with Autographa californica multiply-enveloped Nuclear Polyhedrosis Virus (AcMNPV) was obviously affected by spent medium carry over. On the other hand, initial cell density and Multiplicities of Infection (MOI) have minimal influence on infectivity percentage. 2004-12-08 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/3063/1/50_%28C5-4%29.pdf Yap, Wei Ney and Abdul-Rahman, Badarulhisam and Abdul-Aziz, Azila (2004) Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS). In: National Postgraduate Colloquium, 8-9 December 2004, The Gurnay Resort Hotel & Residence, Penang.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Yap, Wei Ney
Abdul-Rahman, Badarulhisam
Abdul-Aziz, Azila
Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
description An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected with Autographa californica multiply-enveloped Nuclear Polyhedrosis Virus (AcMNPV) was obviously affected by spent medium carry over. On the other hand, initial cell density and Multiplicities of Infection (MOI) have minimal influence on infectivity percentage.
format Conference or Workshop Item
author Yap, Wei Ney
Abdul-Rahman, Badarulhisam
Abdul-Aziz, Azila
author_facet Yap, Wei Ney
Abdul-Rahman, Badarulhisam
Abdul-Aziz, Azila
author_sort Yap, Wei Ney
title Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
title_short Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
title_full Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
title_fullStr Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
title_full_unstemmed Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
title_sort cell culture optmization for the baculovirus expression vector system (bevs)
publishDate 2004
url http://eprints.utm.my/id/eprint/3063/1/50_%28C5-4%29.pdf
http://eprints.utm.my/id/eprint/3063/
_version_ 1643643727351119872
score 13.18916

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