A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food
Formaldehyde is a known carcinogen which may cause cancer when accumulated in the body. This study showed the results of a formaldehyde biosensor which was fabricated by nanogold doping in a poly-2-hydroxy ethyl methacrylate (p-HEMA) membrane. The biocatalysts used for the biosensor were 1.0% ferroc...
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my.utm.305312019-09-22T07:25:38Z http://eprints.utm.my/id/eprint/30531/ A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food Sundari, Rita Hadibarata, Tony Lee, Yook Heng Ahmad, Musa QD Chemistry Formaldehyde is a known carcinogen which may cause cancer when accumulated in the body. This study showed the results of a formaldehyde biosensor which was fabricated by nanogold doping in a poly-2-hydroxy ethyl methacrylate (p-HEMA) membrane. The biocatalysts used for the biosensor were 1.0% ferrocene mediator and alcohol oxidase which was then deposited on a carbon screen-printed electrode. 2,2-Dimethoxy-2-phenyl-acetophenone (DMPP) was applied to the membrane as a polymerization agent. The amperometric method was employed with a phosphate buffer solution (pH = 7.2). The optimum potential was selected to be 0.3 V which obtained good linear calibration (R2 = 0.99) for a range of 0.02-0.16 mM formaldehyde (n = 4). The RSD (Relative Standard Deviation) and LOD (Limit of Detection) were found to be 5.62% and 0.007 mM formaldehyde, respectively. The fabricated biosensor successfully detected formaldehyde in selected fresh foodstuffs (tauhu, meatballs, shrimp and dried and wet fish) and the results were well correlated with the NASH standard method. Academic Journals Inc. 2012 Article PeerReviewed Sundari, Rita and Hadibarata, Tony and Lee, Yook Heng and Ahmad, Musa (2012) A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food. Trends in Applied Sciences Research, 7 (9). pp. 737-747. ISSN 1819-3579 http://dx.doi.org/10.3923/tasr.2012.737.747 DOI:10.3923/tasr.2012.737.747 |
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QD Chemistry Sundari, Rita Hadibarata, Tony Lee, Yook Heng Ahmad, Musa A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food |
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Formaldehyde is a known carcinogen which may cause cancer when accumulated in the body. This study showed the results of a formaldehyde biosensor which was fabricated by nanogold doping in a poly-2-hydroxy ethyl methacrylate (p-HEMA) membrane. The biocatalysts used for the biosensor were 1.0% ferrocene mediator and alcohol oxidase which was then deposited on a carbon screen-printed electrode. 2,2-Dimethoxy-2-phenyl-acetophenone (DMPP) was applied to the membrane as a polymerization agent. The amperometric method was employed with a phosphate buffer solution (pH = 7.2). The optimum potential was selected to be 0.3 V which obtained good linear calibration (R2 = 0.99) for a range of 0.02-0.16 mM formaldehyde (n = 4). The RSD (Relative Standard Deviation) and LOD (Limit of Detection) were found to be 5.62% and 0.007 mM formaldehyde, respectively. The fabricated biosensor successfully detected formaldehyde in selected fresh foodstuffs (tauhu, meatballs, shrimp and dried and wet fish) and the results were well correlated with the NASH standard method. |
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Article |
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Sundari, Rita Hadibarata, Tony Lee, Yook Heng Ahmad, Musa |
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Sundari, Rita Hadibarata, Tony Lee, Yook Heng Ahmad, Musa |
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Sundari, Rita |
title |
A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food |
title_short |
A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food |
title_full |
A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food |
title_fullStr |
A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food |
title_full_unstemmed |
A new biosensor based on nanogold doping in p-HEMA alcohol oxidase detects formaldehyde in fresh food |
title_sort |
new biosensor based on nanogold doping in p-hema alcohol oxidase detects formaldehyde in fresh food |
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Academic Journals Inc. |
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2012 |
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http://eprints.utm.my/id/eprint/30531/ http://dx.doi.org/10.3923/tasr.2012.737.747 |
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