Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples
This study describes an electrokinetic supercharging for online preconcentration capillary electrophoresis (CE) technique of secbumeton in water samples. Important CE separation and preconcentration conditions, such as concentration and pH of the background electrolyte, applied voltage and ultraviol...
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my.utm.289882022-01-31T08:38:16Z http://eprints.utm.my/id/eprint/28988/ Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples Soeung, Rasmey Semail, Nadhiratul Farihin Wan Omar, Wan Adnan Mohamad Zain, Nur Nadhirah Miskam, Mazidatulakmam Wong, Yong Foo Abdul Keyon, Aemi Syazwani Ramachandran, Muggundha Raoov Kamaruzaman, Sazlinda Yahaya, Noorfatimah QD Chemistry This study describes an electrokinetic supercharging for online preconcentration capillary electrophoresis (CE) technique of secbumeton in water samples. Important CE separation and preconcentration conditions, such as concentration and pH of the background electrolyte, applied voltage and ultraviolet wavelength, type and injection time of the terminating electrolyte, and injection time of the leading electrolyte and sample were investigated and optimized. The optimum conditions involved hydrodynamic injection of leading electrolyte (100 mM sodium chloride, 30 s, 50 mbar), electrokinetic injection of the sample as high as 250 s (at +7 kV voltage), and hydrodynamic injection of terminating electrol7te (100 mM Tris buffer, 40 s, 50 mbar). This strategy enhanced secbumeton detection sensitivity up to 3847-fold and 226-fold when compared with hydrodynamic and electrokinetic injection, respectively, providing a limit of detection as low as 0.03 μg L-1with good repeatability (relative standard deviation < 4%, n = 5). Wide linear range (0.1-500 μg L-1) with good linearity (R2= 0.9997) was obtained. The limit of detection was adequate for the analysis of secbumeton in water samples with concentrations lower than its maximum residual limit (0.1 μg L-1). The developed method was applied to environmental water samples, and recoveries were between 85.7 and 105.6%. Penerbit UKM 2020-05 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/28988/1/AemiSyazwani2020_SimpleandSensitiveElectrokineticSupercharging.pdf Soeung, Rasmey and Semail, Nadhiratul Farihin and Wan Omar, Wan Adnan and Mohamad Zain, Nur Nadhirah and Miskam, Mazidatulakmam and Wong, Yong Foo and Abdul Keyon, Aemi Syazwani and Ramachandran, Muggundha Raoov and Kamaruzaman, Sazlinda and Yahaya, Noorfatimah (2020) Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples. Sains Malaysiana, 49 (5). pp. 979-988. ISSN 0126-6039 http://dx.doi.org/10.17576/jsm-2020-4905-02 DOI:10.17576/jsm-2020-4905-02 |
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QD Chemistry Soeung, Rasmey Semail, Nadhiratul Farihin Wan Omar, Wan Adnan Mohamad Zain, Nur Nadhirah Miskam, Mazidatulakmam Wong, Yong Foo Abdul Keyon, Aemi Syazwani Ramachandran, Muggundha Raoov Kamaruzaman, Sazlinda Yahaya, Noorfatimah Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
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This study describes an electrokinetic supercharging for online preconcentration capillary electrophoresis (CE) technique of secbumeton in water samples. Important CE separation and preconcentration conditions, such as concentration and pH of the background electrolyte, applied voltage and ultraviolet wavelength, type and injection time of the terminating electrolyte, and injection time of the leading electrolyte and sample were investigated and optimized. The optimum conditions involved hydrodynamic injection of leading electrolyte (100 mM sodium chloride, 30 s, 50 mbar), electrokinetic injection of the sample as high as 250 s (at +7 kV voltage), and hydrodynamic injection of terminating electrol7te (100 mM Tris buffer, 40 s, 50 mbar). This strategy enhanced secbumeton detection sensitivity up to 3847-fold and 226-fold when compared with hydrodynamic and electrokinetic injection, respectively, providing a limit of detection as low as 0.03 μg L-1with good repeatability (relative standard deviation < 4%, n = 5). Wide linear range (0.1-500 μg L-1) with good linearity (R2= 0.9997) was obtained. The limit of detection was adequate for the analysis of secbumeton in water samples with concentrations lower than its maximum residual limit (0.1 μg L-1). The developed method was applied to environmental water samples, and recoveries were between 85.7 and 105.6%. |
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Soeung, Rasmey Semail, Nadhiratul Farihin Wan Omar, Wan Adnan Mohamad Zain, Nur Nadhirah Miskam, Mazidatulakmam Wong, Yong Foo Abdul Keyon, Aemi Syazwani Ramachandran, Muggundha Raoov Kamaruzaman, Sazlinda Yahaya, Noorfatimah |
author_facet |
Soeung, Rasmey Semail, Nadhiratul Farihin Wan Omar, Wan Adnan Mohamad Zain, Nur Nadhirah Miskam, Mazidatulakmam Wong, Yong Foo Abdul Keyon, Aemi Syazwani Ramachandran, Muggundha Raoov Kamaruzaman, Sazlinda Yahaya, Noorfatimah |
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Soeung, Rasmey |
title |
Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
title_short |
Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
title_full |
Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
title_fullStr |
Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
title_full_unstemmed |
Simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
title_sort |
simple and sensitive electrokinetic supercharging in capillary electrophoresis for online preconcentration and separation of secbumeton in water samples |
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Penerbit UKM |
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2020 |
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http://eprints.utm.my/id/eprint/28988/1/AemiSyazwani2020_SimpleandSensitiveElectrokineticSupercharging.pdf http://eprints.utm.my/id/eprint/28988/ http://dx.doi.org/10.17576/jsm-2020-4905-02 |
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