Design, synthesis and activity study of tyrosinase encapsulated silica aerogel (TESA) biosensor for phenol removal in aqueous solution

• Main Authors: Sani, Suriani, Mohd. Muhid, Mohd. Nazlan, Hamdan, Halimaton
• Format: Article
• Published: Springer Science+Business Media LLC 2011
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/28875/; http://dx.doi.org/10.1007/s10971-011-2454-3

Tyrosinase encapsulated silica aerogel (TESA) was synthesized via an alcohol-free colloidal sol–gel route at room temperature and at neutral pH. Characterization on TESA indicated that 98% of enzyme was effectively loaded and located inside the aerogel network. TESA without solvent extraction showed higher tyrosinase activity than TESA extracted by amyl acetate/acetone (v/v:1/1). Stability of tyrosinase in TESA was enhanced towards extreme temperature, acidic and basic conditions. Optimization study indicates that 500 U enzyme/g silica aerogel; aged for 2 days, showed superior performance in the oxidation of catechol. The activity of TESA was remarkably enhanced; which was active at a wider temperature (up to 80 °C) and pH range (4–9). In contrast, free tyrosinase was totally inactive at these pH values and temperature >55 °C. TESA successfully removed about 90% of phenol in aqueous solution after 3 h of contact time with excellent reusability.