Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA

PCR amplification of ribosomal DNA was used to identify a phenol-degrading yeast strain. Theinternal transcribed spacer regions of the yeast was amplified using universal primers ITSI and ITS4. Based on a BLASTN search of genBank, the complete sequences of ITSI-%.*S rDNA-ITS2 regions and portions of...

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Main Authors: Husin, Aishah, Mohd. Tuah, Piakong, Abdul Rashid, Noor Aini, Yahya, Adibah, Md. Salleh, Madihah, Syed Mohd. Rafeii, Sharifah Norhafizah
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Published: Borneo Science, Universiti Malaysia Sabah 2004
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Online Access:http://eprints.utm.my/id/eprint/28193/
http://wwwsst.ums.edu.my/data/file/VaAb18j4kC8a.pdf
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spelling my.utm.281932022-01-31T08:37:52Z http://eprints.utm.my/id/eprint/28193/ Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA Husin, Aishah Mohd. Tuah, Piakong Abdul Rashid, Noor Aini Yahya, Adibah Md. Salleh, Madihah Syed Mohd. Rafeii, Sharifah Norhafizah QH301 Biology PCR amplification of ribosomal DNA was used to identify a phenol-degrading yeast strain. Theinternal transcribed spacer regions of the yeast was amplified using universal primers ITSI and ITS4. Based on a BLASTN search of genBank, the complete sequences of ITSI-%.*S rDNA-ITS2 regions and portions of 18S and 28S for the purified DNA products of RETL-Crl shared 98% similarity with Candida tropicalis. The nucleotide swquences of the C. tropicalis RETL-Crl was submitted to the GenBank database under the accession number AY725426. Borneo Science, Universiti Malaysia Sabah 2004-06 Article PeerReviewed Husin, Aishah and Mohd. Tuah, Piakong and Abdul Rashid, Noor Aini and Yahya, Adibah and Md. Salleh, Madihah and Syed Mohd. Rafeii, Sharifah Norhafizah (2004) Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA. The Journal of Science & Technology, 15 . pp. 15-22. ISSN 2231-9085 http://wwwsst.ums.edu.my/data/file/VaAb18j4kC8a.pdf
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QH301 Biology
spellingShingle QH301 Biology
Husin, Aishah
Mohd. Tuah, Piakong
Abdul Rashid, Noor Aini
Yahya, Adibah
Md. Salleh, Madihah
Syed Mohd. Rafeii, Sharifah Norhafizah
Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA
description PCR amplification of ribosomal DNA was used to identify a phenol-degrading yeast strain. Theinternal transcribed spacer regions of the yeast was amplified using universal primers ITSI and ITS4. Based on a BLASTN search of genBank, the complete sequences of ITSI-%.*S rDNA-ITS2 regions and portions of 18S and 28S for the purified DNA products of RETL-Crl shared 98% similarity with Candida tropicalis. The nucleotide swquences of the C. tropicalis RETL-Crl was submitted to the GenBank database under the accession number AY725426.
format Article
author Husin, Aishah
Mohd. Tuah, Piakong
Abdul Rashid, Noor Aini
Yahya, Adibah
Md. Salleh, Madihah
Syed Mohd. Rafeii, Sharifah Norhafizah
author_facet Husin, Aishah
Mohd. Tuah, Piakong
Abdul Rashid, Noor Aini
Yahya, Adibah
Md. Salleh, Madihah
Syed Mohd. Rafeii, Sharifah Norhafizah
author_sort Husin, Aishah
title Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA
title_short Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA
title_full Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA
title_fullStr Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA
title_full_unstemmed Molecular identification of Candida tropicalis RETL-Cr1 By PCR amplification of Ribosomal DNA
title_sort molecular identification of candida tropicalis retl-cr1 by pcr amplification of ribosomal dna
publisher Borneo Science, Universiti Malaysia Sabah
publishDate 2004
url http://eprints.utm.my/id/eprint/28193/
http://wwwsst.ums.edu.my/data/file/VaAb18j4kC8a.pdf
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score 13.160551