The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta®
Current study provides an efficient screening system for transformed plant of Citrullus lanatus cv. Round Dragon harboring bar gene. The untransformed 5-day-old cotyledon explants were cultured on the shoot-inducing media supplemented with Basta® (0.2, 0.5, 1.0, 2.0 and 3.0 mg L-1) and without Basta...
Saved in:
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Asian Network for Scientific Information
2010
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/26526/1/FahrulZamanHuyop_2010TheSensitivityofPlantTissueCulture.pdf http://eprints.utm.my/id/eprint/26526/ http://scialert.net/abstract/?doi=ijar.2010.11.18 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.utm.26526 |
---|---|
record_format |
eprints |
spelling |
my.utm.265262018-11-09T08:09:17Z http://eprints.utm.my/id/eprint/26526/ The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® Huyop, Fahrul Zaman K., Ganasan Q Science Current study provides an efficient screening system for transformed plant of Citrullus lanatus cv. Round Dragon harboring bar gene. The untransformed 5-day-old cotyledon explants were cultured on the shoot-inducing media supplemented with Basta® (0.2, 0.5, 1.0, 2.0 and 3.0 mg L-1) and without Basta® for 3 weeks and subcultured on fresh shoot-inducing media with the same media composition for another 3 weeks. The shoot growth on the cotyledon decreased, as the Basta® concentration increased. A complete inhibition of shoot growth was observed on growth medium supplemented with 2.0 and 3.0 mg L-1 of Basta®, respectively. For ex vitro condition, untransformed healthy plant leaves (derived from acclimatized in vitro plantlets) were leaf painted with an aqueous solution of Basta® at the concentration of 0.001, 0.01 and 0.1% (v/v) using writing brush. The sensitivity of untransformed plant tissues were evaluated based on tissue browning and necrosis due to herbicidal damage. Healthy plant leaves subjected to leaf painting assay showed serious necrotic within 3 days at the concentration of 0.1% (v/v) of Basta®. An efficient herbicide Basta® selection mode has been established via in vitro and ex vitro conditions of untransformed Citrullus lanatus cv. Round Dragon. Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/26526/1/FahrulZamanHuyop_2010TheSensitivityofPlantTissueCulture.pdf Huyop, Fahrul Zaman and K., Ganasan (2010) The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta®. International Journal of Agricultural Research, 5 (1). 11 - 18. ISSN 1816-4987 http://scialert.net/abstract/?doi=ijar.2010.11.18 DOI:10.3923/ijar.2010.11.18 |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
language |
English |
topic |
Q Science |
spellingShingle |
Q Science Huyop, Fahrul Zaman K., Ganasan The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
description |
Current study provides an efficient screening system for transformed plant of Citrullus lanatus cv. Round Dragon harboring bar gene. The untransformed 5-day-old cotyledon explants were cultured on the shoot-inducing media supplemented with Basta® (0.2, 0.5, 1.0, 2.0 and 3.0 mg L-1) and without Basta® for 3 weeks and subcultured on fresh shoot-inducing media with the same media composition for another 3 weeks. The shoot growth on the cotyledon decreased, as the Basta® concentration increased. A complete inhibition of shoot growth was observed on growth medium supplemented with 2.0 and 3.0 mg L-1 of Basta®, respectively. For ex vitro condition, untransformed healthy plant leaves (derived from acclimatized in vitro plantlets) were leaf painted with an aqueous solution of Basta® at the concentration of 0.001, 0.01 and 0.1% (v/v) using writing brush. The sensitivity of untransformed plant tissues were evaluated based on tissue browning and necrosis due to herbicidal damage. Healthy plant leaves subjected to leaf painting assay showed serious necrotic within 3 days at the concentration of 0.1% (v/v) of Basta®. An efficient herbicide Basta® selection mode has been established via in vitro and ex vitro conditions of untransformed Citrullus lanatus cv. Round Dragon. |
format |
Article |
author |
Huyop, Fahrul Zaman K., Ganasan |
author_facet |
Huyop, Fahrul Zaman K., Ganasan |
author_sort |
Huyop, Fahrul Zaman |
title |
The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
title_short |
The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
title_full |
The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
title_fullStr |
The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
title_full_unstemmed |
The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
title_sort |
sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® |
publisher |
Asian Network for Scientific Information |
publishDate |
2010 |
url |
http://eprints.utm.my/id/eprint/26526/1/FahrulZamanHuyop_2010TheSensitivityofPlantTissueCulture.pdf http://eprints.utm.my/id/eprint/26526/ http://scialert.net/abstract/?doi=ijar.2010.11.18 |
_version_ |
1643647784802320384 |
score |
13.19449 |