Poly(Styrene-Divinyl Benzene)-Based Monolithic Column For High Performance Liquid Chromatography

• Main Authors: Abd. Aziz, Norsyariza, Abu Naim, Ahmedy, Sanagi, Mohd. Marsin, Wan Ibrahim, Wan Aini
• Format: Monograph
• Language: English
• Published: SPS, UTM 2006
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/1756/1/2006_paper_norsyariza_abd_aziz_for_RPCES.pdf; http://eprints.utm.my/id/eprint/1756/

Poly(styrene-divinyl benzene) (PS-DVB) monoliths were synthesized by in-situ polymerization in a 2.1 mm × 150 mm high performance liquid chromatography, (HPLC) column. The physical properties ofthe monolith were studied by FTIR and SEM. IR spectra of the monolith confirmed the formation of PS-DVB whereas SEM analysis shows that the PS-DVB monolith gave the cauliflower structure. The performance of the synthesized column was evaluated by HPLC and compared with commercial PS-DVB column. The separations were carried out using 4 test compounds; benzaldehyde, nitrobenzene,phenol and toluene. Although the monolith column was less efficient than commercial packed PS-DVB column, the monolith gives faster analysis time.