Cover Image

Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production

During the screening study, a total of 60 different Streptomyces species were isolated from soil samples collected from different areas in Egypt. Streptomyces sp. NRC-35was selected for its high ß-lactamase inhibitor activity mediated by the production of clavulanic acid. This newly isolated strain...

Full description

Saved in:
Bibliographic Details
Main Authors: Awad , Hassan M., El-Shahed , Kamal Y. I., El-Nakkadi, Abd El-Monaem M.
Format: Article
Language:English
Published: IDOSI Publications 2009
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/13868/2/12.pdf
http://eprints.utm.my/id/eprint/13868/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.13868
record_format eprints
spelling my.utm.138682017-02-08T01:09:57Z http://eprints.utm.my/id/eprint/13868/ Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production Awad , Hassan M. El-Shahed , Kamal Y. I. El-Nakkadi, Abd El-Monaem M. QD Chemistry During the screening study, a total of 60 different Streptomyces species were isolated from soil samples collected from different areas in Egypt. Streptomyces sp. NRC-35was selected for its high ß-lactamase inhibitor activity mediated by the production of clavulanic acid. This newly isolated strain was able to produce up to 65 mg L-1 clavulanic acid. The taxonomical properties of the strain were examined according to International Streptomyces Project (ISP) scheme. The primary identification of this isolate was based on spore morphology and cell wall chemo-type. Furthermore, culture physiological characteristics of the isolated strain, Str. NRC-35 were compared to other reference strains belong to Streptomyces species. The analysis of nucleotide sequence of the 16s Rrna indicated similiarity binary level of 91% with Streptomyces species. IDOSI Publications 2009 Article PeerReviewed text/html en http://eprints.utm.my/id/eprint/13868/2/12.pdf Awad , Hassan M. and El-Shahed , Kamal Y. I. and El-Nakkadi, Abd El-Monaem M. (2009) Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production. World Applied Sciences Journal, 7 (5). pp. 637-646. ISSN 1818-4952
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Awad , Hassan M.
El-Shahed , Kamal Y. I.
El-Nakkadi, Abd El-Monaem M.
Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production
description During the screening study, a total of 60 different Streptomyces species were isolated from soil samples collected from different areas in Egypt. Streptomyces sp. NRC-35was selected for its high ß-lactamase inhibitor activity mediated by the production of clavulanic acid. This newly isolated strain was able to produce up to 65 mg L-1 clavulanic acid. The taxonomical properties of the strain were examined according to International Streptomyces Project (ISP) scheme. The primary identification of this isolate was based on spore morphology and cell wall chemo-type. Furthermore, culture physiological characteristics of the isolated strain, Str. NRC-35 were compared to other reference strains belong to Streptomyces species. The analysis of nucleotide sequence of the 16s Rrna indicated similiarity binary level of 91% with Streptomyces species.
format Article
author Awad , Hassan M.
El-Shahed , Kamal Y. I.
El-Nakkadi, Abd El-Monaem M.
author_facet Awad , Hassan M.
El-Shahed , Kamal Y. I.
El-Nakkadi, Abd El-Monaem M.
author_sort Awad , Hassan M.
title Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production
title_short Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production
title_full Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production
title_fullStr Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production
title_full_unstemmed Isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. Nrc-35) for ß-lactamase inhibitor production
title_sort isolation, screening and identification of newly isolated soil streptomyces (streptomyces sp. nrc-35) for ß-lactamase inhibitor production
publisher IDOSI Publications
publishDate 2009
url http://eprints.utm.my/id/eprint/13868/2/12.pdf
http://eprints.utm.my/id/eprint/13868/
_version_ 1643646286135558144
score 13.214268

Similar Items