Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs
A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman...
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The Korean Society for Applied Microbiology
2009
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my.utm.132392017-02-15T00:58:50Z http://eprints.utm.my/id/eprint/13239/ Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs Abdel-Fattah, Y. R. El-Enshasy, Hesham Ali Soliman, Nadia A. El-Gendi, Hebah TP Chemical technology A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman design was applied. Among 15 variables tested, the pH, peptone, and incubation time were selected based on their high positive significant effect on the protease activity. A near-optimum medium formulation was then obtained that increased the protease yield by more than 5-fold. Thereafter, the response surface methodology (RSM) was adopted to acquire the best process conditions among the selected variables, where a 3-level Box-Behnken design was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the protease activity. The optimal combination of the major medium constituents for alkaline protease production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows: pH of 9.5, 2% peptone, and incubation time of 60 h. The predicted optimum alkaline protease activity was 3,213 U/ml/min, which was 6.4 times the activity with the basal medium. The Korean Society for Applied Microbiology 2009 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/13239/1/YRAbdelFattah2009_BioprocessDevelopmentForProductionOfAlkaline%20Protease.pdf Abdel-Fattah, Y. R. and El-Enshasy, Hesham Ali and Soliman, Nadia A. and El-Gendi, Hebah (2009) Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs. Journal of Microbiology and Biotechnology, 19 (4). pp. 378-386. ISSN 10177825 |
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TP Chemical technology Abdel-Fattah, Y. R. El-Enshasy, Hesham Ali Soliman, Nadia A. El-Gendi, Hebah Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs |
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A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman design was applied. Among 15 variables tested, the pH, peptone, and incubation time were selected based on their high positive significant effect on the protease activity. A near-optimum medium formulation was then obtained that increased the protease yield by more than 5-fold. Thereafter, the response surface methodology (RSM) was adopted to acquire the best process conditions among the selected variables, where a 3-level Box-Behnken design was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the protease activity. The optimal combination of the major medium constituents for alkaline protease production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows: pH of 9.5, 2% peptone, and incubation time of 60 h. The predicted optimum alkaline protease activity was 3,213 U/ml/min, which was 6.4 times the activity with the basal medium. |
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Article |
author |
Abdel-Fattah, Y. R. El-Enshasy, Hesham Ali Soliman, Nadia A. El-Gendi, Hebah |
author_facet |
Abdel-Fattah, Y. R. El-Enshasy, Hesham Ali Soliman, Nadia A. El-Gendi, Hebah |
author_sort |
Abdel-Fattah, Y. R. |
title |
Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs |
title_short |
Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs |
title_full |
Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs |
title_fullStr |
Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs |
title_full_unstemmed |
Bioprocess development for production of alkaline protease by bacillus pseudofirmus Mn6 through statistical experimental designs |
title_sort |
bioprocess development for production of alkaline protease by bacillus pseudofirmus mn6 through statistical experimental designs |
publisher |
The Korean Society for Applied Microbiology |
publishDate |
2009 |
url |
http://eprints.utm.my/id/eprint/13239/1/YRAbdelFattah2009_BioprocessDevelopmentForProductionOfAlkaline%20Protease.pdf http://eprints.utm.my/id/eprint/13239/ |
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