Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC
An efficient method for the simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers was developed by CD-modified MEKC using a dual mixture of neutral CDs as chiral selector. Three neutral CDs namely hydroxypropylb- ß-CD, hydroxypropyl-?-CD, and ?-CD were tested a...
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my.utm.131052011-07-18T07:40:12Z http://eprints.utm.my/id/eprint/13105/ Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC Wan Ibrahim, Wan Aini A. Warno, Susanti Aboul-Enein, Hassan Y. Hermawan, Dadan Sanagi, Mohd Marsin QD Chemistry An efficient method for the simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers was developed by CD-modified MEKC using a dual mixture of neutral CDs as chiral selector. Three neutral CDs namely hydroxypropylb- ß-CD, hydroxypropyl-?-CD, and ?-CD were tested as chiral selectors at different concentrations ranging from 10, 20, 30 and 40 mM, but enantiomers of the studied fungicides were not completely separated. The best dual chiral recognition mode for the simultaneous separation of cyproconazole, bromuconazole, and diniconazole enantiomers was achieved with a mixture of 27 mM hydroxypropyl-ß-CD and 3mM hydroxypropyl-?-CD in 25 mM phosphate buffer (pH 3.0) containing 40 mM SDS to which methanol-acetonitrile (10%:5% v/v) was added as organic modifiers. The best separation was based on the appearance of 10 peaks simultaneously, with good resolution (Rs 1.1-15.9), and peak efficiency (N>200 000). Good repeatabilities in the migration time, peak area, and peak height were obtained in terms of RSD ranging from (0.72 to 1.06)%, (0.39 to 3.49)%, and (1.90 to 4.84)%, respectively. John Wiley & Sons Ltd. 2009-06 Article PeerReviewed Wan Ibrahim, Wan Aini and A. Warno, Susanti and Aboul-Enein, Hassan Y. and Hermawan, Dadan and Sanagi, Mohd Marsin (2009) Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC. Electrophoresis, 30 (11). 1976 -1982. ISSN 0173-0835 http://dx.doi.org/10.1002/elps.200800499 doi:10.1002/elps.200800499 |
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QD Chemistry Wan Ibrahim, Wan Aini A. Warno, Susanti Aboul-Enein, Hassan Y. Hermawan, Dadan Sanagi, Mohd Marsin Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC |
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An efficient method for the simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers was developed by CD-modified MEKC using a dual mixture of neutral CDs as chiral selector. Three neutral CDs namely hydroxypropylb- ß-CD, hydroxypropyl-?-CD, and ?-CD were tested as chiral selectors at different concentrations ranging from 10, 20, 30 and 40 mM, but enantiomers of the studied fungicides were not completely separated. The best dual chiral recognition mode for the simultaneous separation of cyproconazole, bromuconazole, and diniconazole enantiomers was achieved with a mixture of 27 mM hydroxypropyl-ß-CD and 3mM hydroxypropyl-?-CD in 25 mM phosphate buffer (pH 3.0) containing 40 mM SDS to which methanol-acetonitrile (10%:5% v/v) was added as organic modifiers. The best separation was based on the appearance of 10 peaks simultaneously, with good resolution (Rs 1.1-15.9), and peak efficiency (N>200 000). Good repeatabilities in the migration time, peak area, and peak height were obtained in terms of RSD ranging from (0.72 to 1.06)%, (0.39 to 3.49)%, and (1.90 to 4.84)%, respectively. |
format |
Article |
author |
Wan Ibrahim, Wan Aini A. Warno, Susanti Aboul-Enein, Hassan Y. Hermawan, Dadan Sanagi, Mohd Marsin |
author_facet |
Wan Ibrahim, Wan Aini A. Warno, Susanti Aboul-Enein, Hassan Y. Hermawan, Dadan Sanagi, Mohd Marsin |
author_sort |
Wan Ibrahim, Wan Aini |
title |
Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC |
title_short |
Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC |
title_full |
Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC |
title_fullStr |
Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC |
title_full_unstemmed |
Simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by CD-modified MEKC |
title_sort |
simultaneous enantioseparation of cyproconazole, bromuconazole, and diniconazole enantiomers by cd-modified mekc |
publisher |
John Wiley & Sons Ltd. |
publishDate |
2009 |
url |
http://eprints.utm.my/id/eprint/13105/ http://dx.doi.org/10.1002/elps.200800499 |
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1643646119470694400 |
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13.159267 |