Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography
Phenylarsonic compounds are extensively used as feed additives to promote growth and to control disease in the poultry industry. A method was conducted that allowed the analysis of arsenic animal feed additives by ion pairing-reversed phase HPLC (IP-RP-HPLC) with a UV detection using an octadecylsil...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2001
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| Online Access: | http://eprints.utm.my/id/eprint/1289/1/JT35C6.pdf http://eprints.utm.my/id/eprint/1289/ http://www.penerbit.utm.my/onlinejournal/35/C/JT35C6.pdf |
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| Summary: | Phenylarsonic compounds are extensively used as feed additives to promote growth and to control disease in the poultry industry. A method was conducted that allowed the analysis of arsenic animal feed additives by ion pairing-reversed phase HPLC (IP-RP-HPLC) with a UV detection using an octadecylsilylsilica column. The separation of anionic arsenic compounds [3-nitro-4-hydroxyphenylarsonic acid (3-NHPAA), p-arsanilic acid (p-ASA) and phenylarsonic acid (PA)] was accomplished using 5 mM tetrabutylammonium chloride as the ion pairing reagent in
aqueous mobile phase containing 3 mM malonic acid and 5% methanol at a flow rate of 1.4 mL min–1. The influence of mobile phase pH on the separation was studied; the optimum pH found was 5.85. The detection limit obtained for 3-NHPAA was 0.43 ng As.
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