Cover Image

Isolation and cloning of human NQO1 promoter in pGL3 basic vector

Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductas...

Full description

Saved in:
Bibliographic Details
Main Author: Chinigarzadeh, Asma
Format: Thesis
Published: 2010
Subjects:
RZ Other systems of medicine
Online Access:http://eprints.utm.my/id/eprint/12287/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.12287
record_format eprints
spelling my.utm.122872011-04-14T09:44:43Z http://eprints.utm.my/id/eprint/12287/ Isolation and cloning of human NQO1 promoter in pGL3 basic vector Chinigarzadeh, Asma RZ Other systems of medicine Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductase: a flavoprotein responsible for catalyzing two-electron reduction and detoxification of quinones and their derivatives. In this study the 2123 bp of the 5` upstream of the first transcription start site was successfully isolated. Based on the bioinformatic program, several regulatory regions such as Antioxidant Response Element (ARE) may be responsible for the direct regulation of polyphenols on this enzyme. It was predicted at -477 from the first transcription start site. Upon isolation, this fragment was used in a cloning process into the pGL3 Basic vector and transformed to E.coli DH5? competent cells 2010-12 Thesis NonPeerReviewed Chinigarzadeh, Asma (2010) Isolation and cloning of human NQO1 promoter in pGL3 basic vector. Masters thesis, Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic RZ Other systems of medicine
spellingShingle RZ Other systems of medicine
Chinigarzadeh, Asma
Isolation and cloning of human NQO1 promoter in pGL3 basic vector
description Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductase: a flavoprotein responsible for catalyzing two-electron reduction and detoxification of quinones and their derivatives. In this study the 2123 bp of the 5` upstream of the first transcription start site was successfully isolated. Based on the bioinformatic program, several regulatory regions such as Antioxidant Response Element (ARE) may be responsible for the direct regulation of polyphenols on this enzyme. It was predicted at -477 from the first transcription start site. Upon isolation, this fragment was used in a cloning process into the pGL3 Basic vector and transformed to E.coli DH5? competent cells
format Thesis
author Chinigarzadeh, Asma
author_facet Chinigarzadeh, Asma
author_sort Chinigarzadeh, Asma
title Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_short Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_full Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_fullStr Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_full_unstemmed Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_sort isolation and cloning of human nqo1 promoter in pgl3 basic vector
publishDate 2010
url http://eprints.utm.my/id/eprint/12287/
_version_ 1643645911849500672
score 13.164666

Similar Items