Extraction and identification of compounds from parkia speciosa seeds by supercritical carbon dioxide

• Main Authors: Che Yunus, Mohd. Azizi, Salman, Z., Nik Norulain, N. A., Mohd. Omar, A. K.
• Format: Article
• Language: English
• Published: Faculty of Chemical and Natural Resource Engineering, UTM 2008
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/12279/1/CYMohdAzizi2008_ExtractionandIdentificationofCompoundsFromParkia.pdf; http://eprints.utm.my/id/eprint/12279/

Parkia speciosa seeds chemical constituents that were obtained from Supercritical Carbon Dioxide (SC-C02) extractions were analysed by Gas Chromatography-Time of Flight Mass Spectrometry (GCrrOF-MS). The SC-C02 extraction was conducted at the interaction of temperature and pressure as; 313 Kl20.68 MPa; 353 Kl20.68 MPa; 313 KlS5. 16 MPa and 353 KI 55.16 MPa in the regime of 50 minutes extraction time. The analysis of compound was based on percentage of similarity and peak area of more than 75% and 0.1% respectively. Propanoic acid, 3, 3'-thiobis - didodecyl ester was present with highest percentage area in most sample condition. Other main compounds were linoleic acid chloride, palmitic acid, linoleic acid, myristic acid, arachidonic acid, undecanoic acid and 2-Hexyl-I-decanol. Terpenoids compounds of ~-sitosterol and squalene were identified at all conditions, while some other compounds such as stigmasterol, lupeol and campesterol were identified only at certain condition. The analysis found 77 compounds in all of the extracted samples. The combination in chromatographic separation with an identification technique as in GCrrOF-MS has made it possible to detect the variability obtained by different SC-C02 extraction condition and separation of different chemical compounds in Parkia speciosa seeds.