Glucose tolerance of Clostridium acetobutylicum fermentation in the anaerobic system

• Main Authors: Fauzi, Basirah, Mohd. Nawawi, Mohd. Ghazali, Fauzi, Roslinda, Mohd. Ismail, Nurul Izzati, Mohd. Noor, Siti Fatimah, Yahaya, Izat, Suhaimy, Syazwan Hanani Meriam, Roslan, Muhammad Sufi
• Format: Article
• Language: English
• Published: North Carolina State University 2023
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/108303/1/MohdGhazali2023_GlucoseToleranceofClostridiumacetobutylicum.pdf; http://eprints.utm.my/108303/; http://dx.doi.org/10.15376/biores.18.4.7915-7922

Solvent-producing Clostridium acetobutylicum was purified and used in an acetone-butanol-ethanol (ABE) fermentation process. The objective of this study is to design a fermentation medium for the synthesis of butanol and determine the ideal glucose concentration for appropriate microbe ingestion. The fermentation medium was incubated at 37 °C for up to 90 h before inoculation while being sparged with nitrogen gas under anaerobic conditions. Based on the optical density of fermentation media, the growth rate was also monitored. At 60 g/L of glucose, which was the optimum condition for fermentation, the process followed a log phase pattern until the death phase, with the largest growth taking place between 10 h and 50 h after incubation. The C. acetobutylicum steadily consumed the glucose content, reaching its maximal consumption with only around 12 g/L remaining. In contrast to acetone and ethanol, which produced the highest concentrations at 6.4 g/L and 5.2 g/L, respectively, butanol productions were seen appropriately, with the greatest concentration yielding 11.2 g/L of butanol. This shows that C. acetobutylicum expressed its active metabolism for up to 60 g/L and further increase of glucose content will deteriorate the performance of butanol production.