Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm.
Background: Exploring genetic diversity in traits of interest and utilizing it to breed high yielding, disease-resistant and high market value varieties is the prime objective of plant breeders. Unfortunately, genetic diversity in most of the crops has declined due to selection and reutilization of...
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2023
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my.utm.1067752024-07-28T06:37:51Z http://eprints.utm.my/106775/ Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. Mahpara, Shahzadi Fatima, Lariab Gul, Ammara Ullah, Rehmat Muneer, Muhammad Bhatti, Muhammad Ashraf Iqbal, Javed Ahmed, Affan Fatima, Tehseen Ijaz, Muhammad Usman Alharbi, Sulaiman Ali Alrasheed, Wafa A. TP Chemical technology Background: Exploring genetic diversity in traits of interest and utilizing it to breed high yielding, disease-resistant and high market value varieties is the prime objective of plant breeders. Unfortunately, genetic diversity in most of the crops has declined due to selection and reutilization of already adapted germplasm. Chemical mutagens are widely used to create novel changes in germplasm; however, these have been rarely tested for chili germplasm in Pakistan. Methods: We used ethyl methane sulfonate (EMS) and sodium azide (SA) to induce mutations for obtaining novel alterations in morphological and yield-related traits of four chili genotypes (i.e., ‘Mexi’, ‘Dandi Cut’, ‘Nageena’ and ‘Talhari’). Firstly, concentrations of both mutagens were calibrated, i.e., 1.5 % EMS and 0.5 % SA. Afterwards, seeds of four chili cultivars were treated and grown in peat trays. As the nursery was established, seedlings were transferred to media (sand: silt: clay + 1 kg well-decomposed FYM) after 30 days of germination. Different mutants were isolated based on differential morphological and phenotypic expressions. Results: It was found that efficiency of EMS was greater than SA and more variants were noticed in case of EMS-treated populations. Genetic analysis showed that almost all the traits were affected by mutagens in the M1 generations although the stability of isolated mutants was not checked in later generations. All the traits showed higher estimates of heritability but in some traits genetic advance was non-significant. Conclusion: It is recommended that isolated mutants could be used as rich genetic diversity source to breed new varieties. Furthermore, EMS proved a suitable mutagen for targeted mutations. Elsevier B.V. 2023-02 Article PeerReviewed application/pdf en http://eprints.utm.my/106775/1/HeshamAliElEnhasy2023_ExploringtheEfficiencyofEthylMethaneSulfonateEMSandSodium.pdf Mahpara, Shahzadi and Fatima, Lariab and Gul, Ammara and Ullah, Rehmat and Muneer, Muhammad and Bhatti, Muhammad Ashraf and Iqbal, Javed and Ahmed, Affan and Fatima, Tehseen and Ijaz, Muhammad Usman and Alharbi, Sulaiman Ali and Alrasheed, Wafa A. (2023) Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. Journal of King Saud University - Science, 35 (2). pp. 1-8. ISSN 1018-3647 http://dx.doi.org/10.1016/j.jksus.2022.102507 DOI:10.1016/j.jksus.2022.102507 |
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TP Chemical technology Mahpara, Shahzadi Fatima, Lariab Gul, Ammara Ullah, Rehmat Muneer, Muhammad Bhatti, Muhammad Ashraf Iqbal, Javed Ahmed, Affan Fatima, Tehseen Ijaz, Muhammad Usman Alharbi, Sulaiman Ali Alrasheed, Wafa A. Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. |
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Background: Exploring genetic diversity in traits of interest and utilizing it to breed high yielding, disease-resistant and high market value varieties is the prime objective of plant breeders. Unfortunately, genetic diversity in most of the crops has declined due to selection and reutilization of already adapted germplasm. Chemical mutagens are widely used to create novel changes in germplasm; however, these have been rarely tested for chili germplasm in Pakistan. Methods: We used ethyl methane sulfonate (EMS) and sodium azide (SA) to induce mutations for obtaining novel alterations in morphological and yield-related traits of four chili genotypes (i.e., ‘Mexi’, ‘Dandi Cut’, ‘Nageena’ and ‘Talhari’). Firstly, concentrations of both mutagens were calibrated, i.e., 1.5 % EMS and 0.5 % SA. Afterwards, seeds of four chili cultivars were treated and grown in peat trays. As the nursery was established, seedlings were transferred to media (sand: silt: clay + 1 kg well-decomposed FYM) after 30 days of germination. Different mutants were isolated based on differential morphological and phenotypic expressions. Results: It was found that efficiency of EMS was greater than SA and more variants were noticed in case of EMS-treated populations. Genetic analysis showed that almost all the traits were affected by mutagens in the M1 generations although the stability of isolated mutants was not checked in later generations. All the traits showed higher estimates of heritability but in some traits genetic advance was non-significant. Conclusion: It is recommended that isolated mutants could be used as rich genetic diversity source to breed new varieties. Furthermore, EMS proved a suitable mutagen for targeted mutations. |
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Mahpara, Shahzadi Fatima, Lariab Gul, Ammara Ullah, Rehmat Muneer, Muhammad Bhatti, Muhammad Ashraf Iqbal, Javed Ahmed, Affan Fatima, Tehseen Ijaz, Muhammad Usman Alharbi, Sulaiman Ali Alrasheed, Wafa A. |
author_facet |
Mahpara, Shahzadi Fatima, Lariab Gul, Ammara Ullah, Rehmat Muneer, Muhammad Bhatti, Muhammad Ashraf Iqbal, Javed Ahmed, Affan Fatima, Tehseen Ijaz, Muhammad Usman Alharbi, Sulaiman Ali Alrasheed, Wafa A. |
author_sort |
Mahpara, Shahzadi |
title |
Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. |
title_short |
Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. |
title_full |
Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. |
title_fullStr |
Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. |
title_full_unstemmed |
Exploring the efficiency of Ethyl Methane Sulfonate (EMS) and Sodium Azide (SA) to induce mutation in chili (Capsicum annuum L.) germplasm. |
title_sort |
exploring the efficiency of ethyl methane sulfonate (ems) and sodium azide (sa) to induce mutation in chili (capsicum annuum l.) germplasm. |
publisher |
Elsevier B.V. |
publishDate |
2023 |
url |
http://eprints.utm.my/106775/1/HeshamAliElEnhasy2023_ExploringtheEfficiencyofEthylMethaneSulfonateEMSandSodium.pdf http://eprints.utm.my/106775/ http://dx.doi.org/10.1016/j.jksus.2022.102507 |
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1805880865980416000 |
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13.18916 |