Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion
Tetraselmis chuii is a potential microalgae that is in consideration for producing bioethanol owing to its large content of carbohydrates. The glucose production from T. chuii through an enzymatic process with cellulase and xylanase (pretreatment process) and α-amylase and glucoamylase (saccharifica...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Royal Society of Chemistry
2023
|
Subjects: | |
Online Access: | http://eprints.utm.my/106711/1/KojiIwamoto2023_MechanismandKineticModelofMicroalgalEnzymatic.pdf http://eprints.utm.my/106711/ http://dx.doi.org/10.1039/d3ra01556d |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.utm.106711 |
---|---|
record_format |
eprints |
spelling |
my.utm.1067112024-07-17T07:13:10Z http://eprints.utm.my/106711/ Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion Padil, Padil Meilana Dharma Putra, Meilana Dharma Putra Muslikhin Hidayat, Muslikhin Hidayat Rina Sri Kasiamdari, Rina Sri Kasiamdari Anisa Mutamima, Anisa Mutamima Iwamoto, Koji Muhammad Arif Darmawan, Muhammad Arif Darmawan Misri Gozan, Misri Gozan Q Science (General) TK Electrical engineering. Electronics Nuclear engineering Tetraselmis chuii is a potential microalgae that is in consideration for producing bioethanol owing to its large content of carbohydrates. The glucose production from T. chuii through an enzymatic process with cellulase and xylanase (pretreatment process) and α-amylase and glucoamylase (saccharification process) was studied. The mechanism of the enzymatic process was developed and the kinetic models were then evaluated. For the pretreatment process, enzymes with 30% concentration reacted at 30 °C for 40 min resulted in 35.9% glucose yield. For the saccharification process, the highest glucose yield of 90.03% was obtained using simultaneous α-amylase (0.0006%) and glucoamylase (0.01%) enzymes at 55 °C and for 40 min. The kinetic models fitted well with the experimental data. The model also revealed that the saccharification process performed better than the pretreatment process with a higher kinetic constant and lower activation energy. The proposed kinetic model plays an important role in implementing processes at a larger scale. Royal Society of Chemistry 2023-06-17 Article PeerReviewed application/pdf en http://eprints.utm.my/106711/1/KojiIwamoto2023_MechanismandKineticModelofMicroalgalEnzymatic.pdf Padil, Padil and Meilana Dharma Putra, Meilana Dharma Putra and Muslikhin Hidayat, Muslikhin Hidayat and Rina Sri Kasiamdari, Rina Sri Kasiamdari and Anisa Mutamima, Anisa Mutamima and Iwamoto, Koji and Muhammad Arif Darmawan, Muhammad Arif Darmawan and Misri Gozan, Misri Gozan (2023) Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion. RSC Advances, 13 (31). pp. 21403-21413. ISSN 2046-2069 http://dx.doi.org/10.1039/d3ra01556d DOI:10.1039/d3ra01556d |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
language |
English |
topic |
Q Science (General) TK Electrical engineering. Electronics Nuclear engineering |
spellingShingle |
Q Science (General) TK Electrical engineering. Electronics Nuclear engineering Padil, Padil Meilana Dharma Putra, Meilana Dharma Putra Muslikhin Hidayat, Muslikhin Hidayat Rina Sri Kasiamdari, Rina Sri Kasiamdari Anisa Mutamima, Anisa Mutamima Iwamoto, Koji Muhammad Arif Darmawan, Muhammad Arif Darmawan Misri Gozan, Misri Gozan Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
description |
Tetraselmis chuii is a potential microalgae that is in consideration for producing bioethanol owing to its large content of carbohydrates. The glucose production from T. chuii through an enzymatic process with cellulase and xylanase (pretreatment process) and α-amylase and glucoamylase (saccharification process) was studied. The mechanism of the enzymatic process was developed and the kinetic models were then evaluated. For the pretreatment process, enzymes with 30% concentration reacted at 30 °C for 40 min resulted in 35.9% glucose yield. For the saccharification process, the highest glucose yield of 90.03% was obtained using simultaneous α-amylase (0.0006%) and glucoamylase (0.01%) enzymes at 55 °C and for 40 min. The kinetic models fitted well with the experimental data. The model also revealed that the saccharification process performed better than the pretreatment process with a higher kinetic constant and lower activation energy. The proposed kinetic model plays an important role in implementing processes at a larger scale. |
format |
Article |
author |
Padil, Padil Meilana Dharma Putra, Meilana Dharma Putra Muslikhin Hidayat, Muslikhin Hidayat Rina Sri Kasiamdari, Rina Sri Kasiamdari Anisa Mutamima, Anisa Mutamima Iwamoto, Koji Muhammad Arif Darmawan, Muhammad Arif Darmawan Misri Gozan, Misri Gozan |
author_facet |
Padil, Padil Meilana Dharma Putra, Meilana Dharma Putra Muslikhin Hidayat, Muslikhin Hidayat Rina Sri Kasiamdari, Rina Sri Kasiamdari Anisa Mutamima, Anisa Mutamima Iwamoto, Koji Muhammad Arif Darmawan, Muhammad Arif Darmawan Misri Gozan, Misri Gozan |
author_sort |
Padil, Padil |
title |
Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
title_short |
Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
title_full |
Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
title_fullStr |
Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
title_full_unstemmed |
Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
title_sort |
mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion |
publisher |
Royal Society of Chemistry |
publishDate |
2023 |
url |
http://eprints.utm.my/106711/1/KojiIwamoto2023_MechanismandKineticModelofMicroalgalEnzymatic.pdf http://eprints.utm.my/106711/ http://dx.doi.org/10.1039/d3ra01556d |
_version_ |
1805880856142675968 |
score |
13.188404 |