Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications
Introduction: Pullulan is a biodegradable biopolymer made up of maltotriose subunits that are water-soluble. It has a direct glucan structure comprising α-1,4 and α-1,6 linkages in a 2:1 ratio, which gives it exceptional physical and structural properties, making it a suitable choice for application...
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2023
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my.utm.1054282024-04-28T09:19:19Z http://eprints.utm.my/105428/ Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications Dailin, Daniel Joe Mohd. Izwan Low, Luo Zaini Mohd. Fuzi, Siti Fatimah Zaharah Tong, Woei Yenn Abdul Manas, Nor Hasmaliana Mehnaz, Samina El Enshasy, Hesham Q Science (General) TP Chemical technology Introduction: Pullulan is a biodegradable biopolymer made up of maltotriose subunits that are water-soluble. It has a direct glucan structure comprising α-1,4 and α-1,6 linkages in a 2:1 ratio, which gives it exceptional physical and structural properties, making it a suitable choice for application in biomedical applications. Despite its potential benefits, the price of production is expansive, and productivity is low, which are significant drawbacks. Therefore, the objective of this work is to identify the ideal carbon source for Aureobasidium melanogenum DSM 2404 to produce high pullulans. Methods: In shake flask studies, the effects of different carbon sources, such as maltose, glucose, sucrose, lactose, and xylose, on cell growth and pullulan generation by Aureobasidium melanogenum DSM 2404 were examined. Results: The highest pullulan production was obtained from sucrose (13.38 g L-1) compared to other types of carbon sources used in the fermentation medium. The maximal pullulan production rate of 0.112 [g-1 L-1 h-1] was obtained in sucrose culture. This suggests that sucrose provides favorable conditions for the microorganisms to produce pullulan at a faster rate compared to other sugars. Conclusion: Sucrose was found to be the most efficient carbon source for the synthesis of pullulan using Aureobasidium melanogenum DSM 2404 generating 13.38 g L-1 of pullulan. Universiti Putra Malaysia 2023-08 Article PeerReviewed application/pdf en http://eprints.utm.my/105428/1/DanielJoeDailin2023_PullulanProductionUtilizingVariousCarbon.pdf Dailin, Daniel Joe and Mohd. Izwan Low, Luo Zaini and Mohd. Fuzi, Siti Fatimah Zaharah and Tong, Woei Yenn and Abdul Manas, Nor Hasmaliana and Mehnaz, Samina and El Enshasy, Hesham (2023) Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications. Malaysian Journal of Medicine and Health Sciences, 19 (SUPP9). pp. 263-268. ISSN 1675-8544 http://dx.doi.org/10.47836/mjmhs.19.s9.36 DOI:10.47836/mjmhs.19.s9.36 |
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Q Science (General) TP Chemical technology Dailin, Daniel Joe Mohd. Izwan Low, Luo Zaini Mohd. Fuzi, Siti Fatimah Zaharah Tong, Woei Yenn Abdul Manas, Nor Hasmaliana Mehnaz, Samina El Enshasy, Hesham Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications |
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Introduction: Pullulan is a biodegradable biopolymer made up of maltotriose subunits that are water-soluble. It has a direct glucan structure comprising α-1,4 and α-1,6 linkages in a 2:1 ratio, which gives it exceptional physical and structural properties, making it a suitable choice for application in biomedical applications. Despite its potential benefits, the price of production is expansive, and productivity is low, which are significant drawbacks. Therefore, the objective of this work is to identify the ideal carbon source for Aureobasidium melanogenum DSM 2404 to produce high pullulans. Methods: In shake flask studies, the effects of different carbon sources, such as maltose, glucose, sucrose, lactose, and xylose, on cell growth and pullulan generation by Aureobasidium melanogenum DSM 2404 were examined. Results: The highest pullulan production was obtained from sucrose (13.38 g L-1) compared to other types of carbon sources used in the fermentation medium. The maximal pullulan production rate of 0.112 [g-1 L-1 h-1] was obtained in sucrose culture. This suggests that sucrose provides favorable conditions for the microorganisms to produce pullulan at a faster rate compared to other sugars. Conclusion: Sucrose was found to be the most efficient carbon source for the synthesis of pullulan using Aureobasidium melanogenum DSM 2404 generating 13.38 g L-1 of pullulan. |
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Article |
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Dailin, Daniel Joe Mohd. Izwan Low, Luo Zaini Mohd. Fuzi, Siti Fatimah Zaharah Tong, Woei Yenn Abdul Manas, Nor Hasmaliana Mehnaz, Samina El Enshasy, Hesham |
author_facet |
Dailin, Daniel Joe Mohd. Izwan Low, Luo Zaini Mohd. Fuzi, Siti Fatimah Zaharah Tong, Woei Yenn Abdul Manas, Nor Hasmaliana Mehnaz, Samina El Enshasy, Hesham |
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Dailin, Daniel Joe |
title |
Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications |
title_short |
Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications |
title_full |
Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications |
title_fullStr |
Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications |
title_full_unstemmed |
Pullulan production utilizing various carbon sources by Aureobasidium melanogenum DSM 2404 as biotherapeutic tool in biomedical applications |
title_sort |
pullulan production utilizing various carbon sources by aureobasidium melanogenum dsm 2404 as biotherapeutic tool in biomedical applications |
publisher |
Universiti Putra Malaysia |
publishDate |
2023 |
url |
http://eprints.utm.my/105428/1/DanielJoeDailin2023_PullulanProductionUtilizingVariousCarbon.pdf http://eprints.utm.my/105428/ http://dx.doi.org/10.47836/mjmhs.19.s9.36 |
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