Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis
Biobased ternary nanocomposites can stabilize enzymes for greater stability, catalytic activity and easy recovery. This study aimed to optimize biogenic silica/magnetite/graphene oxide nanocomposite supported Candida rugosa lipase (CRL/SiO2/Fe3O4/GO) for ethyl valerate (EV) synthesis and characteriz...
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my.utm.1005912023-04-30T08:05:26Z http://eprints.utm.my/id/eprint/100591/ Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis Jacob, Adikwu Gowon Abdul Wahab, Roswanira Chandren, Sheela Jumbri, Khairulazhar Wan Mahmood, Wan Mohd. Asyraf QD Chemistry Biobased ternary nanocomposites can stabilize enzymes for greater stability, catalytic activity and easy recovery. This study aimed to optimize biogenic silica/magnetite/graphene oxide nanocomposite supported Candida rugosa lipase (CRL/SiO2/Fe3O4/GO) for ethyl valerate (EV) synthesis and characterize the biocatalysts’ physicochemical properties and operational stability. CRL conjugated-oil palm leaves-derived biogenic SiO2/Fe3O4/GO nanocomposite showed a maximum immobilized protein of 44.13 ± 2.1 mg/g with a specific activity (534.87 ± 9.5 U/mg), than free CRL (=700 U/mg). GL-A-SiO2/Fe3O4/GO exhibited the highest surface area (260.87 m2/g) alongside superior thermal stability in TGA/DTG. XRD revealed an amorphous SiO2 (crystallinity = 26.7%), while Fe3O4 existed as cubic spinel crystal (crystallinity = 90.2%). Taguchi Design-optimization found that CRL/SiO2/Fe3O4/GO best catalyzed the EV synthesis (90.4% in 3 h) at 40 ? using 3 mg/mL of biocatalyst, valeric acid/ethanol molar ratio of 1:2, in 10% (m/v) molecular sieves with stirring in heptane at 200 rpm. EV production was confirmed by FTIR- (C=O: 1738 cm-1 and C–O–C: 1174 cm-1) and GC–MS ([M]+ m/z = 130, C7H14O2). CRL/SiO2/Fe3O4/GO's reusability for 11 successive esterification cycles demonstrated the SiO2/Fe3O4/GO's exceptional hyperactivation and stabilization properties on immobilized CRL. These findings conveyed the SiO2/Fe3O4/GO's efficacy to alter CRL's physicochemical properties and operational stability for catalyzing higher yields EV. Elsevier B.V. 2022 Article PeerReviewed Jacob, Adikwu Gowon and Abdul Wahab, Roswanira and Chandren, Sheela and Jumbri, Khairulazhar and Wan Mahmood, Wan Mohd. Asyraf (2022) Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis. Advanced Powder Technology, 33 (1). pp. 1-18. ISSN 0921-8831 http://dx.doi.org/10.1016/j.apt.2021.11.026 DOI : 10.1016/j.apt.2021.11.026 |
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QD Chemistry Jacob, Adikwu Gowon Abdul Wahab, Roswanira Chandren, Sheela Jumbri, Khairulazhar Wan Mahmood, Wan Mohd. Asyraf Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
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Biobased ternary nanocomposites can stabilize enzymes for greater stability, catalytic activity and easy recovery. This study aimed to optimize biogenic silica/magnetite/graphene oxide nanocomposite supported Candida rugosa lipase (CRL/SiO2/Fe3O4/GO) for ethyl valerate (EV) synthesis and characterize the biocatalysts’ physicochemical properties and operational stability. CRL conjugated-oil palm leaves-derived biogenic SiO2/Fe3O4/GO nanocomposite showed a maximum immobilized protein of 44.13 ± 2.1 mg/g with a specific activity (534.87 ± 9.5 U/mg), than free CRL (=700 U/mg). GL-A-SiO2/Fe3O4/GO exhibited the highest surface area (260.87 m2/g) alongside superior thermal stability in TGA/DTG. XRD revealed an amorphous SiO2 (crystallinity = 26.7%), while Fe3O4 existed as cubic spinel crystal (crystallinity = 90.2%). Taguchi Design-optimization found that CRL/SiO2/Fe3O4/GO best catalyzed the EV synthesis (90.4% in 3 h) at 40 ? using 3 mg/mL of biocatalyst, valeric acid/ethanol molar ratio of 1:2, in 10% (m/v) molecular sieves with stirring in heptane at 200 rpm. EV production was confirmed by FTIR- (C=O: 1738 cm-1 and C–O–C: 1174 cm-1) and GC–MS ([M]+ m/z = 130, C7H14O2). CRL/SiO2/Fe3O4/GO's reusability for 11 successive esterification cycles demonstrated the SiO2/Fe3O4/GO's exceptional hyperactivation and stabilization properties on immobilized CRL. These findings conveyed the SiO2/Fe3O4/GO's efficacy to alter CRL's physicochemical properties and operational stability for catalyzing higher yields EV. |
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Article |
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Jacob, Adikwu Gowon Abdul Wahab, Roswanira Chandren, Sheela Jumbri, Khairulazhar Wan Mahmood, Wan Mohd. Asyraf |
author_facet |
Jacob, Adikwu Gowon Abdul Wahab, Roswanira Chandren, Sheela Jumbri, Khairulazhar Wan Mahmood, Wan Mohd. Asyraf |
author_sort |
Jacob, Adikwu Gowon |
title |
Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
title_short |
Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
title_full |
Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
title_fullStr |
Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
title_full_unstemmed |
Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
title_sort |
physicochemical properties and operational stability of taguchi design-optimized candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis |
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Elsevier B.V. |
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2022 |
url |
http://eprints.utm.my/id/eprint/100591/ http://dx.doi.org/10.1016/j.apt.2021.11.026 |
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13.160551 |