Genome sequence of an uncharted halophilic bacterium Robertkochia marina with deciphering its phosphate solubilizing ability
The wide use of whole-genome sequencing approach in the modern genomic era has opened a great opportunity to reveal the prospective applications of halophilic bacteria. Robertkochia marina CC-AMO-30DT is one of the halophilic bacteria that was previously taxonomically identified without any inspecti...
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my.uthm.eprints.10822021-08-22T08:33:37Z http://eprints.uthm.edu.my/1082/ Genome sequence of an uncharted halophilic bacterium Robertkochia marina with deciphering its phosphate solubilizing ability Ming, Quan Lam Sye, Jinn Chen Kian, Mau Goh Abd Manan, Fazilah Yahya, Adibah Shamsir, Mohd Shahir QR Microbiology The wide use of whole-genome sequencing approach in the modern genomic era has opened a great opportunity to reveal the prospective applications of halophilic bacteria. Robertkochia marina CC-AMO-30DT is one of the halophilic bacteria that was previously taxonomically identified without any inspection on its biotechnological potential from a genomic aspect. In this study, we present the whole-genome sequence of R. marina and demonstrated the ability of this bacterium in solubilizing phosphate by producing phosphatase. The genome of R. marina has 3.57 Mbp and contains 3107 predicted genes, from which 3044 are protein coding, 52 are non-coding RNAs, and 11 are pseudogenes. Several phosphatases such as alkaline phosphatases and pyrophosphatases were mined from the genome. Further genomic study (phylogenetics, sequence analysis, and functional mechanism) and experimental data suggested that the alkaline phosphatase produced by R. marina could potentially be utilized in promoting plant growth, particularly for plants on saline-based agricultural land. 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/1082/1/J11982_993d3ee6bdcdf3c1c508b8223e7d88cf.pdf Ming, Quan Lam and Sye, Jinn Chen and Kian, Mau Goh and Abd Manan, Fazilah and Yahya, Adibah and Shamsir, Mohd Shahir (2020) Genome sequence of an uncharted halophilic bacterium Robertkochia marina with deciphering its phosphate solubilizing ability. Brazilian Journal of Microbiology, 52. pp. 251-256. (In Press) https://doi.org/10.1007/s42770-020-00401-2 |
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QR Microbiology Ming, Quan Lam Sye, Jinn Chen Kian, Mau Goh Abd Manan, Fazilah Yahya, Adibah Shamsir, Mohd Shahir Genome sequence of an uncharted halophilic bacterium Robertkochia marina with deciphering its phosphate solubilizing ability |
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The wide use of whole-genome sequencing approach in the modern genomic era has opened a great opportunity to reveal the prospective applications of halophilic bacteria. Robertkochia marina CC-AMO-30DT is one of the halophilic bacteria that was previously taxonomically identified without any inspection on its biotechnological potential from a genomic aspect. In this study, we present the whole-genome sequence of R. marina and demonstrated the ability of this bacterium in solubilizing phosphate by producing phosphatase. The genome of R. marina has 3.57 Mbp and contains 3107 predicted genes, from which 3044 are protein coding, 52 are non-coding RNAs, and 11 are pseudogenes. Several phosphatases such as alkaline phosphatases and pyrophosphatases were mined from the genome. Further genomic study (phylogenetics, sequence analysis, and functional mechanism) and experimental data suggested that the alkaline phosphatase produced by R. marina could potentially be utilized in promoting plant growth, particularly for plants on saline-based agricultural land. |
format |
Article |
author |
Ming, Quan Lam Sye, Jinn Chen Kian, Mau Goh Abd Manan, Fazilah Yahya, Adibah Shamsir, Mohd Shahir |
author_facet |
Ming, Quan Lam Sye, Jinn Chen Kian, Mau Goh Abd Manan, Fazilah Yahya, Adibah Shamsir, Mohd Shahir |
author_sort |
Ming, Quan Lam |
title |
Genome sequence of an uncharted
halophilic bacterium Robertkochia
marina with deciphering its phosphate
solubilizing ability |
title_short |
Genome sequence of an uncharted
halophilic bacterium Robertkochia
marina with deciphering its phosphate
solubilizing ability |
title_full |
Genome sequence of an uncharted
halophilic bacterium Robertkochia
marina with deciphering its phosphate
solubilizing ability |
title_fullStr |
Genome sequence of an uncharted
halophilic bacterium Robertkochia
marina with deciphering its phosphate
solubilizing ability |
title_full_unstemmed |
Genome sequence of an uncharted
halophilic bacterium Robertkochia
marina with deciphering its phosphate
solubilizing ability |
title_sort |
genome sequence of an uncharted
halophilic bacterium robertkochia
marina with deciphering its phosphate
solubilizing ability |
publishDate |
2020 |
url |
http://eprints.uthm.edu.my/1082/1/J11982_993d3ee6bdcdf3c1c508b8223e7d88cf.pdf http://eprints.uthm.edu.my/1082/ https://doi.org/10.1007/s42770-020-00401-2 |
_version_ |
1738580817304616960 |
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13.160551 |