Molecular characterization of ornamental fish species (poeciliidae) using mitochondrial dna12s rRNA anD 16S rRNA genes
Mitochondrial DNA (mtDNA) ribosomal genes have been used as molecular markers for fish species identification in many studies. In this study, two mtDNA ribosomal genes namely 12S rRNA and 16S rRNA have been utilized to characterize ornamental fish species from Peocilidae family. Five ornamental f...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2015
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| Subjects: | |
| Online Access: | http://eprints.usm.my/60633/1/SITI%20NORSYAZWANI%20BINTI%20GHAZALI%20-%20%20e.pdf http://eprints.usm.my/60633/ |
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| Summary: | Mitochondrial DNA (mtDNA) ribosomal genes have been used as molecular markers for fish
species identification in many studies. In this study, two mtDNA ribosomal genes namely
12S rRNA and 16S rRNA have been utilized to characterize ornamental fish species from
Peocilidae family. Five ornamental fish species namely Poecilia sphenops, Poecilia
reticulata, Limia vitatta, Xiphophorus hellerii and Xiphophorus maculatus were selected.
PCR amplification was performed and DNA sequencing was carried out in order to study the
genetic relationship of fish species. Phylogenetic tree results showed that two different group
of fish species were found with one groups consists of Limia vitatta, Xiphophorus hellerii and
Xiphophorus maculatus. The second group consists of Poecilia sphenops and Poecilia
reticulata. Interestingly, both mtDNA ribosomal genes showed similar result for neighbor
joining tree. |
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