Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs) expression in murine macrophage cell line J774A.1 stimulated with recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum
Malaria is a disease caused by a Plasmodium parasite that is transmitted by Anopheles mosquitoes. Plasmodium falciparum (P. falciparum') is the most deadly to humans. In Malaysia, malaria is still one of the most important vector-borne diseases, primarily in Sarawak and Sabah. The 19-kDa mer...
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my.usm.eprints.60014 http://eprints.usm.my/60014/ Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs) expression in murine macrophage cell line J774A.1 stimulated with recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum Umar, Mohammad Shahrizal Mohd R5-130.5 General works RA0421 Public health. Hygiene. Preventive Medicine Malaria is a disease caused by a Plasmodium parasite that is transmitted by Anopheles mosquitoes. Plasmodium falciparum (P. falciparum') is the most deadly to humans. In Malaysia, malaria is still one of the most important vector-borne diseases, primarily in Sarawak and Sabah. The 19-kDa merozoite surface protein 1 (MSP-1C) of Plasmodium is a primary candidate for a malaria vaccine as it is highly immunogenic in humans. The inhibition of MSP-1C was previously proposed to be one of the possible mechanisms for the inhibition of merozoite invasion. Interleukin-23 (IL-23) is a cytokine composed of pl9 and p40 subunits which involve in the stimulation of memory CD4+ T cells. The mechanism of IL-23 regulation has been shown to be dependent on signalling molecules known as Mitogen- Activated Protein Kinases (MAPKs) which consist of the p38, extracellular signal-regulated kinase (ERK), and c-Jun N-tenninal kinase (JNK). This study was conducted to determine IL-23 expression by ELISA followed by the detection of signalling pathways activated by Western Blot analysis in macrophages stimulated with rBCG. Macrophage cell line J774A.1 were incubated in DMEM with 10% FBS. The cell was stimulated with BCG and rBCG for 24 and 48 hours. For each incubation time, flasks containing macrophage cultures were scrapped and centrifuged. The supernatant were collected for ELISA analysis while the cell pellets were collected for Western Blot analysis. ELISA result shows that there is an increase of IL-23 expression from 24 to 48 hours; however statistical analysis shows no significant difference. For signalling molecules, only ERK was found to be induced by rBCG stimulation, but not for p38 and JNK. As a conclusion, IL-23 expression is induced in response to rBCG stimulation and it is dependent on ERK signalling molecules. Pusat Pengajian Kesihatan 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/60014/1/MOHAMMAD%20SHAHRIZAL%20MOHD%20UMAR%20-%20e.pdf Umar, Mohammad Shahrizal Mohd (2015) Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs) expression in murine macrophage cell line J774A.1 stimulated with recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum. Project Report. Pusat Pengajian Kesihatan. (Submitted) |
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R5-130.5 General works RA0421 Public health. Hygiene. Preventive Medicine Umar, Mohammad Shahrizal Mohd Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs) expression in murine macrophage cell line J774A.1 stimulated with recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum |
description |
Malaria is a disease caused by a Plasmodium parasite that is transmitted by Anopheles
mosquitoes. Plasmodium falciparum (P. falciparum') is the most deadly to humans. In
Malaysia, malaria is still one of the most important vector-borne diseases, primarily in
Sarawak and Sabah. The 19-kDa merozoite surface protein 1 (MSP-1C) of Plasmodium is a
primary candidate for a malaria vaccine as it is highly immunogenic in humans. The
inhibition of MSP-1C was previously proposed to be one of the possible mechanisms for the
inhibition of merozoite invasion. Interleukin-23 (IL-23) is a cytokine composed of pl9 and
p40 subunits which involve in the stimulation of memory CD4+ T cells. The mechanism of
IL-23 regulation has been shown to be dependent on signalling molecules known as Mitogen-
Activated Protein Kinases (MAPKs) which consist of the p38, extracellular signal-regulated
kinase (ERK), and c-Jun N-tenninal kinase (JNK). This study was conducted to determine
IL-23 expression by ELISA followed by the detection of signalling pathways activated by
Western Blot analysis in macrophages stimulated with rBCG. Macrophage cell line J774A.1
were incubated in DMEM with 10% FBS. The cell was stimulated with BCG and rBCG for
24 and 48 hours. For each incubation time, flasks containing macrophage cultures were
scrapped and centrifuged. The supernatant were collected for ELISA analysis while the cell
pellets were collected for Western Blot analysis. ELISA result shows that there is an increase
of IL-23 expression from 24 to 48 hours; however statistical analysis shows no significant
difference. For signalling molecules, only ERK was found to be induced by rBCG
stimulation, but not for p38 and JNK. As a conclusion, IL-23 expression is induced in
response to rBCG stimulation and it is dependent on ERK signalling molecules. |
format |
Monograph |
author |
Umar, Mohammad Shahrizal Mohd |
author_facet |
Umar, Mohammad Shahrizal Mohd |
author_sort |
Umar, Mohammad Shahrizal Mohd |
title |
Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs)
expression in murine macrophage cell line J774A.1 stimulated with
recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum |
title_short |
Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs)
expression in murine macrophage cell line J774A.1 stimulated with
recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum |
title_full |
Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs)
expression in murine macrophage cell line J774A.1 stimulated with
recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum |
title_fullStr |
Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs)
expression in murine macrophage cell line J774A.1 stimulated with
recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum |
title_full_unstemmed |
Interleukin-23 (IL-23) and mitogen-activated protein kinases (MAPKs)
expression in murine macrophage cell line J774A.1 stimulated with
recombinant bcg (rBCG) clone expressing MSP-1C of Plasmodium falciparum |
title_sort |
interleukin-23 (il-23) and mitogen-activated protein kinases (mapks)
expression in murine macrophage cell line j774a.1 stimulated with
recombinant bcg (rbcg) clone expressing msp-1c of plasmodium falciparum |
publisher |
Pusat Pengajian Kesihatan |
publishDate |
2015 |
url |
http://eprints.usm.my/60014/1/MOHAMMAD%20SHAHRIZAL%20MOHD%20UMAR%20-%20e.pdf http://eprints.usm.my/60014/ |
_version_ |
1797907842454257664 |
score |
13.160551 |