Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells
Astaxanthin is a carotenoid with multiple health benefits including antioxidant and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was generated due to the growing preference for natural pigment. This work aimed to study the astaxanthin-rich X. dendrorhous extracts and...
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my.usm.eprints.59551 http://eprints.usm.my/59551/ Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells Khaw, Shin Yuan R5-920 Medicine (General) Astaxanthin is a carotenoid with multiple health benefits including antioxidant and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was generated due to the growing preference for natural pigment. This work aimed to study the astaxanthin-rich X. dendrorhous extracts and its effect on MCF-7 and MDA-MB- 231 cells. Spectrometric, TLC and HPLC analysis evidenced that mutant M34 produced astaxanthin in higher quantity and purity than the wild type. Mutant astaxanthin extract showed a higher scavenging activity compared to wild type astaxanthin extract in DPPH assay. MTT assays proved that wild type and M34 astaxanthin extracts were not toxic towards the non-cancer origin MCF-10A cells. Wild type and mutant astaxanthin extracts exhibit growth-inhibitory effect in dosedependent manner in MCF-7 and MDA-MB-231 cells. Mutant astaxanthin extract showed a lower IC50 than wild type extract and was more effective in inhibiting both MCF-7 and MDA-MB-231 cells. The IC50 values of wild type and mutant astaxanthin extracts did not exceed the required upper limit of 30 μg/ml. 2022-12 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59551/1/24%20Pages%20from%20KHAW%20SHIN%20YUAN.pdf Khaw, Shin Yuan (2022) Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells. Masters thesis, Perpustakaan Hamzah Sendut. |
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R5-920 Medicine (General) Khaw, Shin Yuan Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
description |
Astaxanthin is a carotenoid with multiple health benefits including antioxidant
and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was
generated due to the growing preference for natural pigment. This work aimed to study
the astaxanthin-rich X. dendrorhous extracts and its effect on MCF-7 and MDA-MB-
231 cells. Spectrometric, TLC and HPLC analysis evidenced that mutant M34
produced astaxanthin in higher quantity and purity than the wild type. Mutant
astaxanthin extract showed a higher scavenging activity compared to wild type
astaxanthin extract in DPPH assay. MTT assays proved that wild type and M34
astaxanthin extracts were not toxic towards the non-cancer origin MCF-10A cells.
Wild type and mutant astaxanthin extracts exhibit growth-inhibitory effect in dosedependent
manner in MCF-7 and MDA-MB-231 cells. Mutant astaxanthin extract
showed a lower IC50 than wild type extract and was more effective in inhibiting both
MCF-7 and MDA-MB-231 cells. The IC50 values of wild type and mutant astaxanthin
extracts did not exceed the required upper limit of 30 μg/ml. |
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Thesis |
author |
Khaw, Shin Yuan |
author_facet |
Khaw, Shin Yuan |
author_sort |
Khaw, Shin Yuan |
title |
Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
title_short |
Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
title_full |
Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
title_fullStr |
Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
title_full_unstemmed |
Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
title_sort |
characterization of astaxanthinrich xanthophyllomyces dendrorhous extract from a hyperproducing mutant and its effects on breast cancer cells |
publishDate |
2022 |
url |
http://eprints.usm.my/59551/1/24%20Pages%20from%20KHAW%20SHIN%20YUAN.pdf http://eprints.usm.my/59551/ |
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