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Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation

Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harve...

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Main Author: Rajan, Kirutika Selva
Format: Thesis
Language:English
Published: 2022
Subjects:
QH1-278.5 Natural history (General)
Online Access:http://eprints.usm.my/59218/1/24%20Pages%20from%20KIRUTIKA%20AP%20SELVA%20RAJAN%20-%20TESIS.pdf
http://eprints.usm.my/59218/
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spelling my.usm.eprints.59218 http://eprints.usm.my/59218/ Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation Rajan, Kirutika Selva QH1-278.5 Natural history (General) Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harvesting. Recently, the latest cryopreservation methods such as V cryo-plate is needed for long-term germplasm conservation. This study aims to develop a V cryo-plate cryopreservation method for L. discolor axillary buds to perform microscopy analysis of cryopreserved explants, determine the water content changes, and genetic stability between control and treated explants. Six (6) parameters have been optimized in this V cryo-plate method which are preculture concentration and duration, types of loading solution, duration on PVS2, thawing temperature, and thawing duration. 2022-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59218/1/24%20Pages%20from%20KIRUTIKA%20AP%20SELVA%20RAJAN%20-%20TESIS.pdf Rajan, Kirutika Selva (2022) Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation. Masters thesis, Perpustakaan Hamzah Sendut.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Rajan, Kirutika Selva
Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
description Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harvesting. Recently, the latest cryopreservation methods such as V cryo-plate is needed for long-term germplasm conservation. This study aims to develop a V cryo-plate cryopreservation method for L. discolor axillary buds to perform microscopy analysis of cryopreserved explants, determine the water content changes, and genetic stability between control and treated explants. Six (6) parameters have been optimized in this V cryo-plate method which are preculture concentration and duration, types of loading solution, duration on PVS2, thawing temperature, and thawing duration.
format Thesis
author Rajan, Kirutika Selva
author_facet Rajan, Kirutika Selva
author_sort Rajan, Kirutika Selva
title Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_short Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_full Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_fullStr Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_full_unstemmed Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
title_sort development of v cryo-plate method of ludisia discolor (ker gawl.) a. rich for long-term germplasm conservation
publishDate 2022
url http://eprints.usm.my/59218/1/24%20Pages%20from%20KIRUTIKA%20AP%20SELVA%20RAJAN%20-%20TESIS.pdf
http://eprints.usm.my/59218/
_version_ 1775623435520573440
score 13.214268

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