Screening and characterization of 10398a polymorphism in invasive breast cancer in Malay population of peninsular Malaysia
The rnitochd~drial DNA (mtDNA) 10398 polymorphism is hypothesised to alter a mitochondrial subunit of the electron transfer chain and is associated with several neurodegenerative disorders and cancers. Methods: In this study, an mtDNApolymorphism at nucleotide position 10398 was screened in 101...
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Kesihatan
2013
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| Subjects: | |
| Online Access: | http://eprints.usm.my/54878/1/MADYA%20DR.%20ZAFARINA%20ZAINUDDIN%20-%20e.pdf http://eprints.usm.my/54878/ |
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| Summary: | The rnitochd~drial DNA (mtDNA) 10398 polymorphism is hypothesised
to alter a mitochondrial subunit of the electron transfer chain and is associated with several
neurodegenerative disorders and cancers.
Methods: In this study, an mtDNApolymorphism at nucleotide position 10398 was screened
in 101 Malay female patients with invasive breast cancer and 90 age-matched healthy female controls
using minisequencing analysis.
Results: The Malay women with the 10398G variant showed a significantly increased risk of
invasive breast cancer (OR = 2.29, 95% CI 1.25-4.20, P = 0.007). lnmlunohistochemistry analysis
was conducted to investigate the effect of this polymorphism on the levels of apoptosis in breast
cancer cells. The level ofBax (a pro-apoptotic protein) expression was significantly higher than that
of Bcl-2 (an anti-apoptotic protein) in patients carrying the G allele (P = 0.016) but not in those
carrying the A allele (P = 0.48).
Conclusion: Based on these findings, we propose that the mtDNA 10398 polymorphism may
be a potential risk marker for breast cancer susceptibility in the Malay population. |
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