The effects of "gamat" on nociceptive processing in the central nervous system

• Main Author: Hayati, Asma
• Format: Monograph
• Language: English
• Published: Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2010
• Subjects: R Medicine
• Online Access: http://eprints.usm.my/53674/1/DR.%20CHE%20BADARIAH%20AB.%20AZIZ-Eprints.pdf; http://eprints.usm.my/53674/

The aims of this study were to determine the analgesic dose ofholothuria extracts and compare the extract effects with dynastat, cyclooxygenase-2 inhibitor, on pain behaviour in rats injected with formalin. In the first part of the study, eighteen Sprague-Dawley male rats (220-300 gram) were allocated into three different groups; saline, holothuria 2 mglkg, and holothuria 4mg/kg. In the second part of the investigation, twenty one Spra~e-Dawley male rats (220-300 gram) were given either holothuria ( 4 mg/kg), saline or dynastat (20mglkg). The treatment was administered intraperitoneally immediately after intraplantar injection of 0.05 ml formalin (1 %). The rats' behaviour was recorded and behaviour data were analysed using repeated measures analysis of variance (ANOV A) with post hoc Scheffe's test and significance level was taken as 0.05. The study has demonstrated that the biphasic response of the formalin test was markedly attenuated in the holothuria 4mglkg group, signifYing inhibited pain behaviour. There was no significant difference between the group receiving dynastat and salil)e from 5 to 50 minutes post formalin injection. At 55 and 60 minutes post formalin, the pain behaviour was significantly reduced for both of the groups receiving holothuria (P< 0.01) and dynastat (P<0.05) when compared to the saline group. Intraperitoneal administration of 4mglkg of holothuria extracts has significantly suppressed the pain behaviour in formalin injected rats compared to dynastat, a cyclooxygenase-2 inhibitor, in both phase I and 2 of formalin test. Results from this investigation throw some light as to the possible use of gamat extract as an analgesic.