The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation
The aim of this study is to investigate the effect of different glucose concentrations on the recycling of co-factor in a non-growing whole-cell Saccharomyces cerevisiae. Besides that, this study also investigates the effect of different glucose concentrations on the biotransformation of 2,6,6-trime...
Saved in:
Main Author: | |
---|---|
Format: | Monograph |
Language: | English |
Published: |
Universiti Sains Malaysia
2017
|
Subjects: | |
Online Access: | http://eprints.usm.my/53330/1/The%20Effect%20Of%20Glucose%20Concentration%20On%20The%20Co-Factor%20Recycling%20In%20A%20Non-Growing%20Whole-Cell%20Saccharomyces%20Cerevisiae-Mediated%20Ketoisophorone%20Biotransformation_Nanthini%20Umapathy_K4_2017.pdf http://eprints.usm.my/53330/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.usm.eprints.53330 |
---|---|
record_format |
eprints |
spelling |
my.usm.eprints.53330 http://eprints.usm.my/53330/ The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation Umapathy, Nanthini T Technology TP155-156 Chemical engineering The aim of this study is to investigate the effect of different glucose concentrations on the recycling of co-factor in a non-growing whole-cell Saccharomyces cerevisiae. Besides that, this study also investigates the effect of different glucose concentrations on the biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione also known as ketoisophorone in a non-growing whole-cell S. cerevisiae. The liquid phase biotransformation was carried out in a shake-flask culture. The conditions of biotransformation are 37 °C, 150 rpm, 5 g/L S. cerevisiae, 0.2 g/L ketoisophorone and varied concentration of glucose (5 g/L, 10g/L, 15 g/L). It was found that level of co-factors were shown at different glucose concentrations. This indicates that co-factor recycling process exist in this reaction.15 g/L of glucose showed the highest value of absorbance which is 0.4100. 5.018×1022 number of glucose molecules are present in 15 g/L glucose. The optimum concentration of glucose for the formation of actinol is 15 g/L. 15 g/L of glucose showed a maximum of 12 mole % of actinol formed. Levodione was the only intermediate formed during the biotransformation. Ketone reduction did not occur due to the inhibition of alcohol dehydrogenase caused by high concentration of NAD+. Besides, ketone reduction has a slower reaction rate as compared to the reduction of carbon-carbon double bond by enoate reductase. Universiti Sains Malaysia 2017-05-03 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53330/1/The%20Effect%20Of%20Glucose%20Concentration%20On%20The%20Co-Factor%20Recycling%20In%20A%20Non-Growing%20Whole-Cell%20Saccharomyces%20Cerevisiae-Mediated%20Ketoisophorone%20Biotransformation_Nanthini%20Umapathy_K4_2017.pdf Umapathy, Nanthini (2017) The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted) |
institution |
Universiti Sains Malaysia |
building |
Hamzah Sendut Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Sains Malaysia |
content_source |
USM Institutional Repository |
url_provider |
http://eprints.usm.my/ |
language |
English |
topic |
T Technology TP155-156 Chemical engineering |
spellingShingle |
T Technology TP155-156 Chemical engineering Umapathy, Nanthini The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation |
description |
The aim of this study is to investigate the effect of different glucose concentrations on the recycling of co-factor in a non-growing whole-cell Saccharomyces cerevisiae. Besides that, this study also investigates the effect of different glucose concentrations on the biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione also known as ketoisophorone in a non-growing whole-cell S. cerevisiae. The liquid phase biotransformation was carried out in a shake-flask culture. The conditions of biotransformation are 37 °C, 150 rpm, 5 g/L S. cerevisiae, 0.2 g/L ketoisophorone and varied concentration of glucose (5 g/L, 10g/L, 15 g/L). It was found that level of co-factors were shown at different glucose concentrations. This indicates that co-factor recycling process exist in this reaction.15 g/L of glucose showed the highest value of absorbance which is 0.4100. 5.018×1022 number of glucose molecules are present in 15 g/L glucose. The optimum concentration of glucose for the formation of actinol is 15 g/L. 15 g/L of glucose showed a maximum of 12 mole % of actinol formed. Levodione was the only intermediate formed during the biotransformation. Ketone reduction did not occur due to the inhibition of alcohol dehydrogenase caused by high concentration of NAD+. Besides, ketone reduction has a slower reaction rate as compared to the reduction of carbon-carbon double bond by enoate reductase. |
format |
Monograph |
author |
Umapathy, Nanthini |
author_facet |
Umapathy, Nanthini |
author_sort |
Umapathy, Nanthini |
title |
The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation |
title_short |
The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation |
title_full |
The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation |
title_fullStr |
The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation |
title_full_unstemmed |
The Effect Of Glucose Concentration On The Co-Factor Recycling In A Non-Growing Whole-Cell Saccharomyces Cerevisiae-Mediated Ketoisophorone Biotransformation |
title_sort |
effect of glucose concentration on the co-factor recycling in a non-growing whole-cell saccharomyces cerevisiae-mediated ketoisophorone biotransformation |
publisher |
Universiti Sains Malaysia |
publishDate |
2017 |
url |
http://eprints.usm.my/53330/1/The%20Effect%20Of%20Glucose%20Concentration%20On%20The%20Co-Factor%20Recycling%20In%20A%20Non-Growing%20Whole-Cell%20Saccharomyces%20Cerevisiae-Mediated%20Ketoisophorone%20Biotransformation_Nanthini%20Umapathy_K4_2017.pdf http://eprints.usm.my/53330/ |
_version_ |
1738511189853339648 |
score |
13.160551 |