The effects of bovine bone scaffold on the microscopic Biological response of human chondrocytes
Introduction In the creation of cartilaginous tissue, the choice of suitable scaffold remains a great challenge. The current available scaffold either natural or synthetic still does not meet the requirement of a scaffold for cartilage tissue engineering. The ideal scaffold provides mechanical s...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2009
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| Subjects: | |
| Online Access: | http://eprints.usm.my/50838/1/DR.%20BAHARUDIN%20BIN%20ABDULLAH-Eprints.pdf http://eprints.usm.my/50838/ |
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| Summary: | Introduction
In the creation of cartilaginous tissue, the choice of suitable scaffold remains a
great challenge. The current available scaffold either natural or synthetic still
does not meet the requirement of a scaffold for cartilage tissue engineering. The
ideal scaffold provides mechanical stability to the individual cell as a construct or
transitional framework before synthesis of new extra cellular matrix.
Objective
The aim of this study was to produce bovine bone as a tissue engineering
construct for cartilage reconstruction and to determine the effects of bovine bone
on biological human chondrocyte in vitro.
Method
Human chondrocytes were cultured and seeded into bovine scaffold with seeding
density of 1 x 105 cells per 100 ul I scaffold and incubated for 24 hours, 2 days, 5
days and 7 days. Proliferation and viability of the cells were measured by
mitochondrial dehydrogenase activity (MTT assay), adhesion study was analyzed
using Scanning Electron Microscopy (SEM) and differentiation study was
analyzed by lmmunoflorescent staining using Confocal Laser Scanning Electron
Microscopy (CLSM). |
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