Dna fragmentation in hela cells treated with Brucea javanica fruits extract
Fruits of Brucea javanica (B. javanica) have been used as traditional medicine in China as it has been shown to have antimalarial, antiplasmodial, antitumor or anticancer and antiamoebic. In this study, methanol extracts of B. javanica fruit (BJF) were treated on human cervical cancer cell (HeLa)...
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
2008
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| Subjects: | |
| Online Access: | http://eprints.usm.my/50706/1/NOOR%20HIDAYU%20BINTI%20PATHNI%20-%2024%20pages.pdf http://eprints.usm.my/50706/ |
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| Summary: | Fruits of Brucea javanica (B. javanica) have been used as traditional medicine in China as
it has been shown to have antimalarial, antiplasmodial, antitumor or anticancer and
antiamoebic. In this study, methanol extracts of B. javanica fruit (BJF) were treated on
human cervical cancer cell (HeLa). Antiproliferative activity of BJF methanol extracts on
HeLa cells showed ICso value of 13.34 J.l.g/ml. After that, TUNEL assay (Terminal
Deoxynucleotide Transferase dUTP Nick End Labeling) was done to detect the apoptosis
rate of BJF methanol extract on HeLa cells. The apoptosis rate for 24 h, 48 h and 72 h were
13.8%, 17.8% and 22.9% respectively. The percentage of apoptosis rate was time
dependent. These results proposed that BJF methanol extracts exhibits antiproliferative
effect on HeLa cells by inducing apoptosis. Therefore, it may be a potential candidate for
antiproliferative agent for the treatment of human cervical cancer cells. |
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