DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts

Plants have a long history of use in the treatment of cancer. Plants have played an important role as a source of effective anti-cancer agents, and it is significant that over 60% of currently used anti-cancer agents are derived from natural sources, including plants, marine organisms and micro-o...

Full description

Saved in:
Bibliographic Details
Main Author: Abdullah, Mastura
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2008
Subjects:
Online Access:http://eprints.usm.my/49745/1/MASTURA%20BINTI%20ABDULLAH-24%20pages.pdf
http://eprints.usm.my/49745/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usm.eprints.49745
record_format eprints
spelling my.usm.eprints.49745 http://eprints.usm.my/49745/ DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts Abdullah, Mastura R Medicine Plants have a long history of use in the treatment of cancer. Plants have played an important role as a source of effective anti-cancer agents, and it is significant that over 60% of currently used anti-cancer agents are derived from natural sources, including plants, marine organisms and micro-organisms. Methylene blue assay is done to determine the ICso value of Syzygium Polyanthum. IC50 is a concentration that needed to 50% of the cell population death. Plant extract with different concentrations are tested to cells depends on time. After treatment, live cell will take up methylene blue dye and the result will be analyzed by using ELISA reader at 660nm. The effectiveness of the plant extract will be determined based on ICso value. TUNNEL assay is done to detect the rate of apoptosis in HeLa cells treated with S.polyanthum. TUNEL is actually stood for terminal deoxynucleotide transferase dUTP Nick End Labeling. It is use to detect DNA fragmentation that are produced by apoptotic cells. At the last of this experiment, we are able to detect DNA fragmentation event in HeLa cells treated with S.polyanthum extract. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/49745/1/MASTURA%20BINTI%20ABDULLAH-24%20pages.pdf Abdullah, Mastura (2008) DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts. Other. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Abdullah, Mastura
DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
description Plants have a long history of use in the treatment of cancer. Plants have played an important role as a source of effective anti-cancer agents, and it is significant that over 60% of currently used anti-cancer agents are derived from natural sources, including plants, marine organisms and micro-organisms. Methylene blue assay is done to determine the ICso value of Syzygium Polyanthum. IC50 is a concentration that needed to 50% of the cell population death. Plant extract with different concentrations are tested to cells depends on time. After treatment, live cell will take up methylene blue dye and the result will be analyzed by using ELISA reader at 660nm. The effectiveness of the plant extract will be determined based on ICso value. TUNNEL assay is done to detect the rate of apoptosis in HeLa cells treated with S.polyanthum. TUNEL is actually stood for terminal deoxynucleotide transferase dUTP Nick End Labeling. It is use to detect DNA fragmentation that are produced by apoptotic cells. At the last of this experiment, we are able to detect DNA fragmentation event in HeLa cells treated with S.polyanthum extract.
format Monograph
author Abdullah, Mastura
author_facet Abdullah, Mastura
author_sort Abdullah, Mastura
title DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
title_short DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
title_full DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
title_fullStr DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
title_full_unstemmed DNA fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
title_sort dna fragmentation in hela cancer cell lines treated with syzygium polyanthum extracts
publisher Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
publishDate 2008
url http://eprints.usm.my/49745/1/MASTURA%20BINTI%20ABDULLAH-24%20pages.pdf
http://eprints.usm.my/49745/
_version_ 1709668328919793664
score 13.222552