Development of comparative genomic hybridization(CGH) technique for the study of nasopharyngeal carcinoma(NPC)
NPC is a disease in which malignant cells are formed in the tissue of nasopharynx. It is a highly prevalent disease in Southern China and Southeast Asia including Malaysia. CGH is a molecular cytogenetic technique which is used to identify imbalanced genetic alterations in this malignancy. Twenty...
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| Main Authors: | , , , , , |
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
2009
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| Subjects: | |
| Online Access: | http://eprints.usm.my/48688/1/PROF.%20MADYA%20DR.%20ZILFALIL%20ALWI-OCR.pdf http://eprints.usm.my/48688/ |
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| Summary: | NPC is a disease in which malignant cells are formed in the tissue of
nasopharynx. It is a highly prevalent disease in Southern China and Southeast Asia
including Malaysia. CGH is a molecular cytogenetic technique which is used to identify
imbalanced genetic alterations in this malignancy. Twenty eight samples were obtained.
Out of this; twelve tumors were extracted from twelve NPC biopsies while twelve
references DNA was extracted from twelve normal controls peripheral blood. Tumor
DNA and normal DNA was labeled by nick translation method with green and red
fluorescent dyes. These were hybridized at metaphase chromosomes DNA and
counterstained with DAPL Finally, the image was captured and analyzed. Chromosomal
gains that were found in this study were 4q26, llql3-ql4, 9pl3, 8ql3-q22 and 10q22-
q26 while chromosomal losses were found at region 20p12 and 13q21-q31. We believe
this study has provided the platform for further investigations to locate possible tumorsuppressor
genes and oncogenes in our NPC patients. |
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