Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
Flagella was commonly known as the tail of bacteria. The major structural protein of bacterial flagella is called flagellin. Flagella have a potential to become biodegradable nanotube and important for drug delivery, electronic or semiconductor. There are still lack of research about flagella sep...
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Teknologi Industri
2020
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| Online Access: | http://eprints.usm.my/46941/1/PHYSICAL%20EXTRACTION%20OF%20FLAGELLAVIA%20%28ABSTRACT%29.pdf http://eprints.usm.my/46941/ http://ethesis.usm.my:8080/jspui/ |
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| Summary: | Flagella was commonly known as the tail of bacteria. The major structural protein of
bacterial flagella is called flagellin. Flagella have a potential to become biodegradable
nanotube and important for drug delivery, electronic or semiconductor. There are still
lack of research about flagella separation and purification. In this research, the
morphology of the flagella and basal body for Bacillus subtilis 168 was investigated
by scanning electron microscope and transmission electron microscope. The
characterization of flagella was found that difference between flagella and cell body is
nitrogen (N), magnesium (Mg) and Sulphur (S) by energy-dispersive X-ray
spectroscopy. Statistically designed experiment revealed significant effects of
sonication duration and sonicator output power on the concentration of flagellin after
sonication. The cell body of Bacillus subtilis 168 was started to breaked from 20 %
sonicator output power. |
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