Comparison Of Proteomes Of Avirulent Mycobacterium Tuberculosis H37Ra And Virulent Mycobacterium Tuberculosis H37Rv

• Main Author: Ang, Kai Cheen
• Format: Thesis
• Language: English
• Published: 2011
• Subjects: RS1-441 Pharmacy and materia medica
• Online Access: http://eprints.usm.my/43226/1/ANG%20KAI%20CHEEN.pdf; http://eprints.usm.my/43226/

M. tuberculosis H37Ra, which was discovered in 1935, was derived from the virulent strain H37. In order to identify the factors that are correlated to virulence in M. tuberculosis H37Rv, 2D-gel profile of three virulent strains at culture grown for three time points (14, 28 and 50 days) were compared with its avirulent counterpart M. tuberculosis H37Ra. A total of 250 protein spots were visualized by Coomassie blue staining. The protein profiles of the M. tuberculosis H37Rv and M. tuberculosis H37Ra were highly similar (R2~0.70), thirty one spots were found to be differentially expressed at 14, 28 and 50 days cultures, whereby 14 spots were identified from Tris extract and 17 spots were identified from TLB extract.