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Identification of a potential marker for Brugia malayi infection by Western Blot analysis

Brugia malayi adult antigen was electrophoresed on SDS-PAGE gels and electrophoretically transferred onto PVDF membranes. The membrane strips were incubated with different categories of human sera, followed by successive incubations with blocking solution, monoclonal anti-human IgG4 antibody and per...

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Main Authors: N., Rahmah, A., Noor A'shikin, R., Mehdi, B., Abdullah, A., Khairul Anuar
Format: Conference or Workshop Item
Language:English
Subjects:
RA643-645 Disease (Communicable and noninfectious) and public health
Online Access:http://eprints.usm.my/42634/1/GP...Identification_Of_A_Potential_Marker_For_Brugia_Malayi_Infection_By_Western_Blot_Analysis..OCR...pdf
http://eprints.usm.my/42634/
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spelling my.usm.eprints.42634 http://eprints.usm.my/42634/ Identification of a potential marker for Brugia malayi infection by Western Blot analysis N., Rahmah A., Noor A'shikin R., Mehdi B., Abdullah A., Khairul Anuar RA643-645 Disease (Communicable and noninfectious) and public health Brugia malayi adult antigen was electrophoresed on SDS-PAGE gels and electrophoretically transferred onto PVDF membranes. The membrane strips were incubated with different categories of human sera, followed by successive incubations with blocking solution, monoclonal anti-human IgG4 antibody and peroxidase rabbit anti-mouse IgG antibody; with adequate washings done in between each incubation steps. Chemiluminescence detection was used to develop the blots. Two antigenic epitopes (molecular weights of -67-68 kDa and -54-55 kDA) were found to be present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with positive anti-filarial IgG4 antibodies, some treated patients and some elephantiasis patients. We hypothesized that last two groups are still harbouring live adult worms despite being treated or in the chronic stage respectively. The two epitopes did not simultaneously react with soil-transmitted helminth sera, normal endemic sera and sera of city dwellers.Therefore the simultaneous presence of both epitopes may potentially serve as a marker for Brugia malayi infection. Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42634/1/GP...Identification_Of_A_Potential_Marker_For_Brugia_Malayi_Infection_By_Western_Blot_Analysis..OCR...pdf N., Rahmah and A., Noor A'shikin and R., Mehdi and B., Abdullah and A., Khairul Anuar Identification of a potential marker for Brugia malayi infection by Western Blot analysis. In: Identification of a potential marker for Brugia malayi infection by Western Blot analysis. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RA643-645 Disease (Communicable and noninfectious) and public health
spellingShingle RA643-645 Disease (Communicable and noninfectious) and public health
N., Rahmah
A., Noor A'shikin
R., Mehdi
B., Abdullah
A., Khairul Anuar
Identification of a potential marker for Brugia malayi infection by Western Blot analysis
description Brugia malayi adult antigen was electrophoresed on SDS-PAGE gels and electrophoretically transferred onto PVDF membranes. The membrane strips were incubated with different categories of human sera, followed by successive incubations with blocking solution, monoclonal anti-human IgG4 antibody and peroxidase rabbit anti-mouse IgG antibody; with adequate washings done in between each incubation steps. Chemiluminescence detection was used to develop the blots. Two antigenic epitopes (molecular weights of -67-68 kDa and -54-55 kDA) were found to be present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with positive anti-filarial IgG4 antibodies, some treated patients and some elephantiasis patients. We hypothesized that last two groups are still harbouring live adult worms despite being treated or in the chronic stage respectively. The two epitopes did not simultaneously react with soil-transmitted helminth sera, normal endemic sera and sera of city dwellers.Therefore the simultaneous presence of both epitopes may potentially serve as a marker for Brugia malayi infection.
format Conference or Workshop Item
author N., Rahmah
A., Noor A'shikin
R., Mehdi
B., Abdullah
A., Khairul Anuar
author_facet N., Rahmah
A., Noor A'shikin
R., Mehdi
B., Abdullah
A., Khairul Anuar
author_sort N., Rahmah
title Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_short Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_full Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_fullStr Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_full_unstemmed Identification of a potential marker for Brugia malayi infection by Western Blot analysis
title_sort identification of a potential marker for brugia malayi infection by western blot analysis
url http://eprints.usm.my/42634/1/GP...Identification_Of_A_Potential_Marker_For_Brugia_Malayi_Infection_By_Western_Blot_Analysis..OCR...pdf
http://eprints.usm.my/42634/
_version_ 1662755731517997056
score 13.160551

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