Structural conformation of Bacillus stearothermophilus F1 protease and effect of modification on its thermostability

• Main Authors: Ibrahim, Noor Azlina, Rahman, Raja Noor Zaliha R. Abd., Rahman, Mohd. Basyaruddin Abd., Basri, Mahiran, Salleh, Abu Bakar
• Other Authors: Ahmad, Abdul Latif
• Format: Book Section
• Language: English
• Published: Penerbit Universiti Sains Malaysia 2004
• Subjects: Q179.9-180 Research
• Online Access: http://eprints.usm.my/42518/1/pBIO36.pdf; http://eprints.usm.my/42518/

The extracellular F1 serine protease, produced by a thermophilic Bacillus stearothermophilus F1, has been isolated and characterized as one of a serine protease. F1 protease was stable in the pH range of 8.0 to 10.0, with an optimum activity at pH 9.0. The enzyme was stable for 24h at 70°C (Rahman...