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The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model

Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent biological properties useful in wound management. However, improvement of the physical and chemical properties of the chitosan will alter its biocompatibility. Additionally, chitosan has been reported to be useful in...

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Main Author: Lim , Chin Keong
Format: Thesis
Language:English
Published: 2010
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/42288/1/Lim_Chin_Keong24.pdf
http://eprints.usm.my/42288/
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spelling my.usm.eprints.42288 http://eprints.usm.my/42288/ The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model Lim , Chin Keong R5-920 Medicine (General) Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent biological properties useful in wound management. However, improvement of the physical and chemical properties of the chitosan will alter its biocompatibility. Additionally, chitosan has been reported to be useful in scar intervention. Therefore in this study, the in vitro biocompatibility of the modified chitosan porous skin regenerating templates (CPSRTs) has been evaluated based on cytotoxicity analysis by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, genotoxicity by comet assay, and skin keratinocyte pro-inflammatory cytokine secretions [interleukin-1 (IL-1), tumor necrosis factor- (TNF-) and interleukin-8 (IL-8)], using primary normal human epidermal keratinocyte (pNHEK) cultures. The most biocompatible CPSRT was then investigated for its role in keloid intervention using keratinocyte-fibroblast cocultures. The human collagen type-I, III and V protein expressions were assayed in Western blot analysis. 2010-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42288/1/Lim_Chin_Keong24.pdf Lim , Chin Keong (2010) The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model. PhD thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Lim , Chin Keong
The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
description Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent biological properties useful in wound management. However, improvement of the physical and chemical properties of the chitosan will alter its biocompatibility. Additionally, chitosan has been reported to be useful in scar intervention. Therefore in this study, the in vitro biocompatibility of the modified chitosan porous skin regenerating templates (CPSRTs) has been evaluated based on cytotoxicity analysis by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, genotoxicity by comet assay, and skin keratinocyte pro-inflammatory cytokine secretions [interleukin-1 (IL-1), tumor necrosis factor- (TNF-) and interleukin-8 (IL-8)], using primary normal human epidermal keratinocyte (pNHEK) cultures. The most biocompatible CPSRT was then investigated for its role in keloid intervention using keratinocyte-fibroblast cocultures. The human collagen type-I, III and V protein expressions were assayed in Western blot analysis.
format Thesis
author Lim , Chin Keong
author_facet Lim , Chin Keong
author_sort Lim , Chin Keong
title The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
title_short The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
title_full The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
title_fullStr The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
title_full_unstemmed The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
title_sort cyto-genotoxicity evaluation of biomedical grade chitosan on primary keratinocyte cultures and its role in scar formation pathways using skin coculture model
publishDate 2010
url http://eprints.usm.my/42288/1/Lim_Chin_Keong24.pdf
http://eprints.usm.my/42288/
_version_ 1643710462138777600
score 13.211869

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