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Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei

Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this stu...

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Main Author: Shakrin, Nik Noorul Shakira Mohamed
Format: Thesis
Language:English
Published: 2010
Subjects:
R735-854 Medical education. Medical schools. Research
Online Access:http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf
http://eprints.usm.my/42167/
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spelling my.usm.eprints.42167 http://eprints.usm.my/42167/ Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei Shakrin, Nik Noorul Shakira Mohamed R735-854 Medical education. Medical schools. Research Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye. 2010-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf Shakrin, Nik Noorul Shakira Mohamed (2010) Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R735-854 Medical education. Medical schools. Research
spellingShingle R735-854 Medical education. Medical schools. Research
Shakrin, Nik Noorul Shakira Mohamed
Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
description Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye.
format Thesis
author Shakrin, Nik Noorul Shakira Mohamed
author_facet Shakrin, Nik Noorul Shakira Mohamed
author_sort Shakrin, Nik Noorul Shakira Mohamed
title Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_short Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_full Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_fullStr Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_full_unstemmed Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
title_sort development of dna-based diagnostic tests for the detection of shigella dysenteriae, shigella flexneri and shigella sonnei
publishDate 2010
url http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf
http://eprints.usm.my/42167/
_version_ 1643710427654258688
score 13.160551

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