In Vitro Selection of Rna Aptamers that Specifically Bind to 50kda Outer Membrane Protein of Salmonella Enterica Serovar Typhi

• Main Author: Ramli, Siti Mariam Mawarni
• Format: Thesis
• Language: English
• Published: 2016
• Subjects: R735-854 Medical education. Medical schools. Research
• Online Access: http://eprints.usm.my/40838/1/Dr.__Siti_Mariam_Mawarni_Ramli_TSTZ.pdf; http://eprints.usm.my/40838/

Demam kepialu merupakan penyakit bawaan makanan yang disebabkan oleh jangkitan bakteria Salmonella enterica serovar Typhi (S. Typhi). Demam kepialu masih lagi menjadi satu permasalahan kesihatan awam di kebanyakan negara mundur dan negara yang sedang membangun termasuk Malaysia. Dianggarkan kira-kira 22 juta kes dan 216,000 kematian akibat daripada jangkitan S. Typhi telah dilaporkan di seluruh dunia setiap tahun. Membran protin luar (OMP) spesifik dengan berat molekul pada 50 ribu Dalton (50 kDa), daripada S. Typhi telah dilaporkan berkemungkinan memainkan peranan dalam jangkitan demam kepialu Typhoid fever is a food borne illness caused by the bacteria, Salmonella enterica serovar Typhi (S. Typhi). Typhoid fever remains a public health problem in many of the underdeveloped and developing countries including Malaysia. It has been estimated around 22 million cases and 216,000 related deaths occurred worldwide annually. The 50 kDa outer membrane protein (OMP) of S. Typhi has been considered as a possible candidate that plays role during the infection of typhoid. Therefore 50kDa OMP of S. Typhi was used as target molecule in detecting of S. Typhi. Currently, monoclonal and polyclonal antibodies were widely used in diagnostic kit development for antigen detection from the organisms that caused the disease