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Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays

Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity by in vitro assay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE using in vitro assays. Methods. Standardized human whole blood (WB) clots were incu...

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Main Authors: Elnager, Abuzar, Hassan, Rosline, Idris, Zamzuri, Mustafa, Zulkifli, Arfah, Nadiah Wan, Sulaiman, S. A., Siew, Hua Gan, Abdullah, Wan Zaidah
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2015
Subjects:
BD240-260 Methodology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
Online Access:http://eprints.usm.my/39119/1/Fibrinolytic_Activity_and_Dose-Dependent_Effect.pdf
http://eprints.usm.my/39119/
http://dx.doi.org/10.1155/2015/627471
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spelling my.usm.eprints.39119 http://eprints.usm.my/39119/ Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays Elnager, Abuzar Hassan, Rosline Idris, Zamzuri Mustafa, Zulkifli Arfah, Nadiah Wan Sulaiman, S. A. Siew, Hua Gan Abdullah, Wan Zaidah BD240-260 Methodology RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM Therapeutics. Pharmacology Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity by in vitro assay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE using in vitro assays. Methods. Standardized human whole blood (WB) clots were incubated in either blank controls or different concentrations of CAPE (3.75, 7.50, 15.00, 22.50, and 30.00mM). After 3 hours, D-dimer (DD) levels and WB clot weights were measured for each concentration. Thromboelastography (TEG) parameters were recorded following CAPE incubation, and fibrin morphology was examined under a confocal microscope. Results. Overall, mean DD ( Hindawi Publishing Corporation 2015 Article PeerReviewed application/pdf en http://eprints.usm.my/39119/1/Fibrinolytic_Activity_and_Dose-Dependent_Effect.pdf Elnager, Abuzar and Hassan, Rosline and Idris, Zamzuri and Mustafa, Zulkifli and Arfah, Nadiah Wan and Sulaiman, S. A. and Siew, Hua Gan and Abdullah, Wan Zaidah (2015) Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays. BioMed Research International, 2015 (627471). pp. 1-10. ISSN 2314-6133 http://dx.doi.org/10.1155/2015/627471
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic BD240-260 Methodology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
spellingShingle BD240-260 Methodology
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
Elnager, Abuzar
Hassan, Rosline
Idris, Zamzuri
Mustafa, Zulkifli
Arfah, Nadiah Wan
Sulaiman, S. A.
Siew, Hua Gan
Abdullah, Wan Zaidah
Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays
description Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity by in vitro assay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE using in vitro assays. Methods. Standardized human whole blood (WB) clots were incubated in either blank controls or different concentrations of CAPE (3.75, 7.50, 15.00, 22.50, and 30.00mM). After 3 hours, D-dimer (DD) levels and WB clot weights were measured for each concentration. Thromboelastography (TEG) parameters were recorded following CAPE incubation, and fibrin morphology was examined under a confocal microscope. Results. Overall, mean DD (
format Article
author Elnager, Abuzar
Hassan, Rosline
Idris, Zamzuri
Mustafa, Zulkifli
Arfah, Nadiah Wan
Sulaiman, S. A.
Siew, Hua Gan
Abdullah, Wan Zaidah
author_facet Elnager, Abuzar
Hassan, Rosline
Idris, Zamzuri
Mustafa, Zulkifli
Arfah, Nadiah Wan
Sulaiman, S. A.
Siew, Hua Gan
Abdullah, Wan Zaidah
author_sort Elnager, Abuzar
title Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays
title_short Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays
title_full Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays
title_fullStr Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays
title_full_unstemmed Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester: In Vitro Assays
title_sort fibrinolytic activity and dose-dependent effect of incubating human blood clots in caffeic acid phenethyl ester: in vitro assays
publisher Hindawi Publishing Corporation
publishDate 2015
url http://eprints.usm.my/39119/1/Fibrinolytic_Activity_and_Dose-Dependent_Effect.pdf
http://eprints.usm.my/39119/
http://dx.doi.org/10.1155/2015/627471
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score 13.211869

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