Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

• Main Authors: Omar, Noorsharmimi, Loh, Qiuting, Gee, Jun Tye, Yee, Siew Choong, Noordin, Rahmah, Jörn, Glökler, Lim, Theam Soon
• Format: Article
• Language: English
• Published: MDPI 2014
• Subjects: R735-854 Medical education. Medical schools. Research
• Online Access: http://eprints.usm.my/38675/1/Development_of_an_Antigen-DNAzyme_Based_Probe_for_a_Direct_Antibody-Antigen.pdf; http://eprints.usm.my/38675/; https://doi.org/10.3390/s140100346

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the applic...